AssayWise Letters 2012. Vol. 1(2)

Detection and Bioimaging of Nitric Oxide (NO) Using Multicolor DAX " J2™ Reagents
DAF-2 reagents are frequently used to detect nitric oxide (NO). However, DAF-2 diacetate is spontaneously hydrolyzed in cell culture media. The hydrolyzed DAF-2 is not cell-permeable, thus causing high assay background.…

Intracellular pH Probes
Intracellular pH plays an important modulating role in many cel-lular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, en-docytosis, chemotaxis, cell adhesion, and other cellular processes.…

JC"10™ a Superior JC-1 Replacement
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 μM concentra-tion, JC-1 tends to precipitate in aqueous buffer. JC-10™ has been developed to be an alternative to JC-1 where high dye concentra-tion is desired.…

Labeling Actins with iFluor™-Phalloidin Conjugates
Fluorescent phalloidin derivatives have been used as an important tool in studying of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.…

Lysyl Oxidase (LOX)
Lysyl oxidase (LOX) is an extracellular enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors.These aldehydes are highly reactive, and undergo spontaneous chemical reactions with other lysyl oxidase-derived aldehyde residues, or with unmodified…

Acetylcholinesterase Quantification
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. AChE is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its…

Pyrophosphate Detection
Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Our PhosphoWroks™ Pyrophosphate Assay Kit…

Aldehyde Quantification
Rapid and accurate measurement of aldehydes is an important task for biological research, food industry, chemical research and environmental pollution surveillance. Our Amplite™ Colorimetric Aldehyde Quantitation Kit uses a proprietary dye that generates a chromogenic product upon reacting…

Tide Quenchers, the Best Dark FRET Acceptors Covering the Full Visible and NIR Spectrum
Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency of longer wavelength dyes (such as fluoresceins, rhodamines and cyanines) has limited its use in the development of sensitive fluorogenic FRET probes.Additionally, the absorption spectrum of…

ReadiView™ Biotin
Biotin/avidin complexes are widely applied for a variety of biological detections. Although a large number of biotin-labeled bioconjugates are commercially available, the accurate determina-tion of biotinylation degree (ratio of biotin/biopolymer) is still a great challenge for biochemists.HABA…

RPE"Streptavidin
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.RPE-streptavidin conjugate offered by AAT Bioquest comprises…

Indocyanine Green (ICG)
Indocyanine green (ICG) is a cyanine dye used in medical diagnos-tics. It is used for determining cardiac output, hepatic function, liver blood flow, and ophthalmic angiography. It has a peak spectral absorption close to 800 nm.…

iFluor™ 790
In vivo fluorescence whole body imaging uses a sensitive camera to detect fluorescence emission from fluorophores in small animals.To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely…