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RPE-streptavidin
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. RPE-streptavidin conjugate offered by AAT Bioquest comprises streptavidin (as the biotin-binding protein) with RPE covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining when used in the conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry, microplate reader and microarray platforms. Some of our customers have used this RPE-streptavidin conjugate with Affymetrix's microarray platform, Luminex's XMAP® and XTAG® technologies (e.g., Bio-Rad's Bioplex assay system). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
Fig. 1
absorbance and emission spectra of RPE-Streptavidin in PBS buffer
The absorbance and emission spectra of RPE-Streptavidin (Catalog Number 16900) in PBS buffer (pH 7.2).

This document (03.0023.121001r1) was last updated on Wed Oct 29 2025. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
RPE-streptavidin