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ReadiView™ Biotin

The Chemical structure of ReadiView™ biotin
The Chemical structure of ReadiView™ biotin. RM = reactive moiety for labeling purpose; CT = color tag for calculating biotinylation degree.
Biotin/avidin complexes are widely applied for a variety of biological detections. Although a large number of biotin-labeled bioconjugates are commercially available, the accurate determination of biotinylation degree (ratio of biotin/biopolymer) is still a great challenge for biochemists. HABA is predominantly used for determining the degree of biotinylation (through its absorption with the extinction coefficient = 34,000/M-1cm-1). When a biotin-containing sample is added, the biotin binds strongly to avidin and displaces the weakly bound HABA. The resulting decrease in absorbance relates to the amount of biotin. However there are many factors that affect the accuracy of HABA method, making this method unreliable for many biotin-labeled conjugates.

Our ReadiView™ biotin contains specially designed Color Tag (CT) that makes the biotinylation degree readily accessible by simply calculating the corrected absorption ratio of 280 nm/385 nm. Our specially designed tag has a very minimal effect on the biotin binding affinity, and its absorption maximum is designed to make the tag have minimal quenching effect on most fluorophores used for labeling avidins.

Table 1. Ordering Info for Biotin Products

Product Name
Unit Size
3001Biotin *CAS 58-85-5*1 g
3002Biotin, succinimidyl ester *CAS 35013-72-0*100 mg
3003Biotin ethylenediamine *CAS 216299-38-6*10 mg
3004Biotin cadaverine25 mg
3005Biotin C2 maleimide25 mg
3006Biotin-4-fluorescein *CAS 1032732-74-3*5 mg492518
3007Biotin hydrazide *CAS 66640-86-6*25 mg
3009Biotin-X NTA [Biotin-X nitrilotriacetic acid, potassium salt] *CAS 856661-92-2*1 mg
3010Biotin-X, succinimidyl ester *CAS 72040-63-2*25 mg
3014Biotin PEG2 amine *CAS 138529-46-1*5 mg
3015Biotin PEG2 maleimide *CAS 305372-39-8*5 mg
3016Biotin PEG2 succinimidyl ester25 mg
3017Fluorescein biotin5 mg492515
3019Biotin-PEG3-azide *CAS 945633-30-7*5 mg
3020Biotin Azide5 mg
3021Biotin Alkyne *CAS 1006592-45-5*5 mg
3022Biotin PEG4 succinimidyl ester25 mg
3050ReadiView™ biotin acid25 mg
3053ReadiView™ biotin amine5 mg
3055ReadiView™ biotin hydrazide5 mg
3058ReadiView™ biotin maleimide5 mg
3059ReadiView™ biotin succinimidyl ester5 mg
3110AF532 PEG4 biotin conjugate1 mg534553
5520ReadiLink™ Protein Biotinylation Kit2 Labelings
5521ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*1 kit
5522Amplite® Colorimetric Biotin Quantitation Kit200 Tests500
16729Biotinylated goat anti-mouse IgG (H+L)1 mg
16794Biotinylated goat anti-rabbit IgG (H+L)1 mg
17016Biotin-11-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 86303-25-5*25 nmoles
17017Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 136632-31-0*25 nmoles
17018Biotin-20-dUTP *1 mM in Tris Buffer (pH 7.5)*25 nmoles
20605Cal-520®-Biotin Conjugate5x50 ug492514
17105Biotin-cAMP conjugate100 ug
6220Biotin-dT Phosphoramidite50 umoles
3008Biotin NTA5 mg
3018Biotin PEG 4 Alkyne5 mg
45352Biotin-PEG4-tyramide200 Slides
6222Biotin Phosphoramidite100 umoles
12630Biotin-X IDA1 mg
26050Acridinium Biotin Conjugate1 mg
17100N6-Methyladenosine-Biotin conjugate1 mg
3013Biotin PEG4 amine5 mg
39050Psoralen-PEG3-Biotin1 mg
39052ReadiLink™ Psoralen-A17-Biotin1 mg
17110Bio-16-UTP [Biotin-16-UTP] *10 mM*25 nmoles