AAT Bioquest

Tide Quenchers, the Best Dark FRET Acceptors Covering the Full Visible and NIR Spectrum

Tide Quencher™ Acceptor Dyes, Optimized to Maximize FRET Efficiency

Normalized absorption spectra of Tide Quencher dyes
The normalized absorption spectra of TQ1, 2, 3, 4, 5, 6 and 7.
Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency of longer wavelength dyes (such as fluoresceins, rhodamines and cyanines) has limited its use in the development of sensitive fluorogenic FRET probes. Additionally, the absorption spectrum of DABCYL is environment-sensitive. AAT Bioquest has developed the robust Tide Quencher™ acceptor dyes for the development of longer wavelength FRET probes. These Tide Quencher™ dark FRET acceptors (such as TQ1, TQ2, TQ3, TQ4, TQ5, TQ6 and TQ7) are optimized to pair with our Tide Fluor™ dyes and the classic fluorophores (such as AMCA, EDANS, FAM, TAMRA, HEX, JOE, TET, ROX, Cy3, Cy5 and Cy7). Like our Tide Fluor™ donor dyes, our Tide Quencher™ acceptor dyes are much more cost-effective with comparable or even better performance for your desired biological applications than other similar products on the market.

FRET peptide substrate is digested by a protease
The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.
Besides their broad applications in the development of Molecular Beacon probes, our Tide Quencher™ dyes have also been used to develop various protease substrates such as HIV protease, MMPs and secretases. In some cases, they have demonstrated greatly improved enzyme performance. This may be partly due to the red-shifted absorption spectrum that overlaps better with the emission spectrum of fluoresceins, rhodamines and cyanines. Tide Quencher™ dyes are a great choice for you to eliminate the limitations of classic quenchers. Tide Quencher™ dyes are excellent dark quenchers that are individually optimized to pair with all the popular fluorescent dyes such as fluoresceins, rhodamines and cyanines. Our Tide Quencher™ series of nonfluorescent dyes cover the full visible spectrum with unusually high efficiency. TQ2 has absorption maximum perfectly matching the emission of FAM while TQ3, TQ5 and TQ7 are proven to be the best quencher for Cy3, Cy5 and Cy7.

HIV Protease cleavage of Arg-Glu(5-FAM)-Val-Ser-Phe-Asn-Phe-Pro-Gln-Ile-Thr-Lys(TQ2)-Arg
HIV Protease cleavage of Arg-Glu(5-FAM)-Val-Ser-Phe-Asn-Phe-Pro-Gln-Ile-Thr-Lys(TQ2)-Arg. The substrate is incubated with HIV protease . Upon HIV protease cleavage, the fluorescence of 5-FAM is recovered, and monitored with Ex/Em = 490 nm/520 nm.

The Advantages of Tide Quencher™ Dyes:

  • Most Powerful: TQ dyes enable you to explore the FRET potentials that might be impossible with other quenchers.
  • Versatile Reactive Forms: convenient for self-constructing your desired FRET biomolecules.
  • A Complete Set of Dyes: perfectly match your desired fluorescent donors.
  • Enhanced Value: competitive price with better performance.

Selection of FRET Donors and Acceptors

Recommended FRET pairs
Recommended FRET pairs for developing molecular beacon nucleic acid detection and protease assays*.
FRET can be detected either by the appearance of sensitized fluorescence of the acceptor, by the intensity ratio change of donor/acceptor (if the acceptor is fluorescent), or by the fluorescence decrease of the donor. In the later case, acceptor can be either fluorescent or non-fluorescent. Table 4.1. summarizes our Tide Quencher™ FRET building blocks designed to develop FRET probes for the demanding applications. Based on our in-house research and experience, it is recommended to use the FRET pairs marked in green. The pairs marked in light blue are OK to use, but less efficient. We have proved that these recommended FRET pairs have demonstrated high sensitivity and low background in our protease and nucleic acid detection assays. There are also other factors that you need to consider besides the FRET efficiency, such as pH, multiplexing and buffer interference, etc.

Table 1. Ordering Info For Tide Quencher Products

Product Name
Unit Size
2170Tide Quencher™ 2WS acid [TQ2WS acid]25 mg
2178Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]5 mg
2179Tide Quencher™ 2WS maleimide [TQ2WS maleimide]1 mg
2060Tide Quencher™ 4WS acid [TQ4WS acid]5 mg
2061Tide Quencher™ 4WS amine [TQ4WS amine]1 mg
2062Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*100 mg
2063Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*100 mg
2064Tide Quencher™ 4WS maleimide [TQ4WS maleimide]1 mg
2067Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]1 mg
2068Tide Quencher™ 4WS azide [TQ4WS azide]1 mg
2069Tide Quencher™ 4WS alkyne [TQ4WS alkyne]1 mg
2075Tide Quencher™ 5WS acid [TQ5WS acid]5 mg
2076Tide Quencher™ 5WS amine [TQ5WS amine]1 mg
2077Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å*100 mg
2078Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*100 mg
2079Tide Quencher™ 5WS maleimide [TQ5WS maleimide]1 mg
2081Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]1 mg
2082Tide Quencher™ 5WS azide [TQ5WS azide]1 mg
2083Tide Quencher™ 5WS alkyne [TQ5WS alkyne]1 mg
2090Tide Quencher™ 6WS acid [TQ6WS acid]5 mg
2091Tide Quencher™ 6WS amine [TQ6WS amine]1 mg
2094Tide Quencher™ 6WS maleimide [TQ6WS maleimide]1 mg
2096Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE]1 mg
2097Tide Quencher™ 6WS azide [TQ6WS azide]1 mg
2098Tide Quencher™ 6WS alkyne [TQ6WS alkyne]1 mg
2105Tide Quencher™ 7WS acid [TQ7WS acid]5 mg
2106Tide Quencher™ 7WS amine [TQ7WS amine]1 mg
2109Tide Quencher™ 7WS maleimide [TQ7WS maleimide]1 mg
2111Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]1 mg
2112Tide Quencher™ 7WS azide [TQ7WS azide]1 mg
2113Tide Quencher™ 7WS alkyne [TQ7WS alkyne]1 mg
2188Tide Quencher™ 1 azide [TQ1 azide]5 mg
2189Tide Quencher™ 1 alkyne [TQ1 alkyne]5 mg
2190Tide Quencher™ 1 acid [TQ1 acid]100 mg
2192Tide Quencher™ 1 amine [TQ1 amine]5 mg
2193Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*100 mg
2194Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*100 mg
2196Tide Quencher™ 1 maleimide [TQ1 maleimide]5 mg
2198Tide Quencher™ 1 phosphoramidite [TQ1 phosphoramidite]100 umoles
2199Tide Quencher™ 1 succinimidyl ester [TQ1 SE]25 mg
2200Tide Quencher™ 2 acid [TQ2 acid]100 mg
2202Tide Quencher™ 2 amine [TQ2 amine]5 mg
2203Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å*100 mg
2204Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å*100 mg
2208Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite]100 umoles
2210Tide Quencher™ 2 succinimidyl ester [TQ2 SE]25 mg
2211Tide Quencher™ 2 azide [TQ2 azide]5 mg
2212Tide Quencher™ 2 alkyne [TQ2 alkyne]5 mg
2213Tide Quencher™ 2WS alkyne [TQ2WS alkyne]1 mg
2214Tide Quencher™ 2WS alkyne [TQ2WS alkyne]5 mg
2220Tide Quencher™ 3 acid [TQ3 acid]100 mg
2222Tide Quencher™ 3 amine [TQ3 amine]5 mg
2223Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å*100 mg
2224Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*100 mg
2226Tide Quencher™ 3 maleimide [TQ3 maleimide]5 mg
2227Tide Quencher™ 3WS acid [TQ3WS acid]5 mg
2228Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite]100 umoles
2229Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE]1 mg
2230Tide Quencher™ 3 succinimidyl ester [TQ3 SE]25 mg
2231Tide Quencher™ 3 azide [TQ3 azide]5 mg
2232Tide Quencher™ 3 alkyne [TQ3 alkyne]5 mg