Tide Quenchers, the Best Dark FRET Acceptors Covering the Full Visible and NIR Spectrum
Tide Quencher™ Acceptor Dyes, Optimized to Maximize FRET Efficiency
Besides their broad applications in the development of Molecular Beacon probes, our Tide Quencher™ dyes have also been used to develop various protease substrates such as HIV protease, MMPs and secretases. In some cases, they have demonstrated greatly improved enzyme performance. This may be partly due to the red-shifted absorption spectrum that overlaps better with the emission spectrum of fluoresceins, rhodamines and cyanines. Tide Quencher™ dyes are a great choice for you to eliminate the limitations of classic quenchers. Tide Quencher™ dyes are excellent dark quenchers that are individually optimized to pair with all the popular fluorescent dyes such as fluoresceins, rhodamines and cyanines. Our Tide Quencher™ series of nonfluorescent dyes cover the full visible spectrum with unusually high efficiency. TQ2 has absorption maximum perfectly matching the emission of FAM while TQ3, TQ5 and TQ7 are proven to be the best quencher for Cy3, Cy5 and Cy7.
The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.
HIV Protease cleavage of Arg-Glu(5-FAM)-Val-Ser-Phe-Asn-Phe-Pro-Gln-Ile-Thr-Lys(TQ2)-Arg. The substrate is incubated with HIV protease . Upon HIV protease cleavage, the fluorescence of 5-FAM is recovered, and monitored with Ex/Em = 490 nm/520 nm.
The Advantages of Tide Quencher™ Dyes:
- Most Powerful: TQ dyes enable you to explore the FRET potentials that might be impossible with other quenchers.
- Versatile Reactive Forms: convenient for self-constructing your desired FRET biomolecules.
- A Complete Set of Dyes: perfectly match your desired fluorescent donors.
- Enhanced Value: competitive price with better performance.
Selection of FRET Donors and Acceptors
Table 1. Ordering Info For Tide Quencher Products
|2170||Tide Quencher™ 2WS acid [TQ2WS acid]||25 mg|
|2178||Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]||5 mg|
|2179||Tide Quencher™ 2WS maleimide [TQ2WS maleimide]||1 mg|
|2060||Tide Quencher™ 4WS acid [TQ4WS acid]||5 mg|
|2061||Tide Quencher™ 4WS amine [TQ4WS amine]||1 mg|
|2062||Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*||100 mg|
|2063||Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*||100 mg|
|2064||Tide Quencher™ 4WS maleimide [TQ4WS maleimide]||1 mg|
|2067||Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]||1 mg|
|2068||Tide Quencher™ 4WS azide [TQ4WS azide]||1 mg|