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AAT Bioquest

Aldehyde Quantification

Rapid and accurate measurement of aldehydes is an important task for biological research, food industry, chemical research and environmental pollution surveillance. There are few reagents or assay kits available for quantifying aldehydes. Most of existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. Our Amplite® Colorimetric Aldehyde Quantitation Kit uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. The kit provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm.
Aldehyde dose response
Formaldehyde dose response

Left: Aldehyde dose response was measured in a 96-well clear plate with Amplite® Colorimetric Aldehyde Quantitation Assay Kit. As low as 10 µM (1 nanomol/well) of aldehyde can be detected. Right: Formaldehyde dose response was measured in a 96-well black plate with Amplite® Fluorimetric Aldehyde Quantitation Kit. As low as 1 µM (100 picomoles/well) of formaldehyde can be detected with 15 minutes incubation time (n=3).

 

Table 1. Aldehyde Quantitation Kits Ordering Information

Cat No.
Product Name
Ex (nm)
Em (nm)
Unit Size
10051Amplite® Colorimetric Aldehyde Quantitation Kit550 200 Tests
10053Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*620 200 Tests
10052Amplite® Fluorimetric Aldehyde Quantitation Kit360450200 Tests
10057Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*400510200 Tests