Rapid and accurate measurement of aldehydes is an important task for biological research, food industry, chemical research and environmental pollution surveillance. There are few reagents or assay kits available for quantifying aldehydes. Most of existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. Our Amplite^® Colorimetric Aldehyde Quantitation Kit uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. The kit provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm.