Name: Portelite™ Rapid Fluorimetric Biotin Quantitation Kit *Optimized to Use with CytoCite™ and Qubit™ Fluorometers*
Brand: AAT Bioquest
SKU: 5539
Price: 237 USD
Availability: InStock

Portelite™ Rapid Fluorimetric Biotin Quantitation Kit

Optimized to Use with CytoCite™ and Qubit™ Fluorometers

Portelite™ Rapid Fluorimetric Biotin Quantitation Kit is a fluorimetric assay kit for quantifying biotin and biotin conjugates using CytoCite™ or Qubit™ fluorometers.

Detection Principle: Biotinylite™ Green increases fluorescence upon binding biotin or biotin conjugates.
Signal Response: Fluorescence intensity is proportional to biotin concentration.
Chemistry: Uses the fluorogenic Biotinylite™ Green sensor.
Quantitation Method: Determines biotin from a standard calibration curve.
Instrument Compatibility: Optimized for CytoCite™ and Qubit™ fluorometers at Ex/Em 490/530 nm.

The Biotin Standard Curve was determined using the Portelite™ Rapid Fluorimetric Biotin Quantitation Kit, with measurements taken on a Qubit™ 4 Fluorometer using the blue (470 nm) channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5539	50 tests	Price

Overview

Portelite™ Rapid Fluorimetric Biotin Quantitation Kit is specifically optimized to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite™ and Qubit™ Fluorometer. The kit uses Biotinylite™ Green, a fluorogenic biotin sensor. Biotinylite™ Green is almost non-fluorescent and give strong green fluorescence upon interaction with a biotin or biotin conjugate. The concentration of biotin is proportional to the fluorescence intensity of Biotinylite™ Green. The amount of biotin is determined by comparing a sample’s fluorescence to the predetermined biotin standard curve. This fluorescence-based assay is much more sensitive than the commonly used colorimetric HABA assay. Biotin is a relatively small molecule that is routinely conjugated to antibodies and proteins with minimal interference of their biological activity. The avidin/streptavidin-biotin interaction is the strongest known binding pair between a protein and its ligand. The biotin-avidin interaction has been extensively explored for a variety of biological applications.

References

View all 47 references: Citation Explorer

Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning.

Authors:

Yu, Haibin and Liu, Dandan and Zhang, Yaowen and Tang, Ruijun and Fan, Xunan and Mao, Song and Lv, Lu and Chen, Fang and Qin, Hongtao and Zhang, Zhuohua and van Aalten, Daan M F and Yang, Bing and Yuan, Kai

Journal:

eLife (2024)

Nano-On-Nano: Responsive Nanosubstrate-Mediated Liposome Delivery with High Cellular Uptake Efficiency.

Authors:

Gautam, Bhaskarchand and Luo, Chun-Hao and Gao, Hua-De and Hsiao, Jye-Chian and Tseng, Hsian-Rong and Lee, Hsien-Ming and Yu, Hsiao-Hua

Journal:

ACS applied bio materials (2023): 1611-1620

Biotin conjugates in targeted drug delivery: is it mediated by a biotin transporter, a yet to be identified receptor, or (an)other unknown mechanism(s)?

Authors:

Tripathi, Ravi and Guglani, Anchala and Ghorpade, Rujuta and Wang, Binghe

Journal:

Journal of enzyme inhibition and medicinal chemistry (2023): 2276663

Clickable Photoreactive ATP-Affinity Probe for Global Profiling of ATP-Binding Proteins.

Authors:

Wang, Zhiming and Tan, Jing and Li, Mengxuan and Gao, Can and Li, Wenwen and Xu, Jing and Guo, Changchuan and Chen, Zhenzhen and Cai, Rong

Journal:

Analytical chemistry (2023): 17533-17540

Development of disposable electrode for the detection of mosquito-borne viruses.

Authors:

Nasrin, Fahmida and Khoris, Indra Memdi and Chowdhury, Ankan Dutta and Muttaqein, Sjakurrizal El and Park, Enoch Y

Journal:

Biotechnology journal (2023): e2300125

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	490 nm
Emission	530 nm
Instrument specification(s)	Green Fluorescent Channel

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Page updated on October 26, 2026

Component A: Biotinylite™ Green (10X)	2 Vials (0.5 mL/Vial)
Component B: Biotin Standard (300 µM)	2 Vials (100 µL/Vial)
Component C: Assay Buffer	1 Bottle (25 mL)