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AAT Bioquest

Biotin-cAMP conjugate

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Physical properties
Molecular weight988.11
SolventDMSO
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
988.11
Cyclic adenosine monophosphate (cAMP) is a second messenger used for intracellular signal induction. It is synthesized from adenosine triphosphate (ATP) by enzymes (g-proteins) that are attached to metabotropic receptors and become released when the receptor is activated. Cyclic AMP is involved in the regulation of glycogen, sugar, and lipid metabolism. This cAMP-biotin conjugate is an excellent probe that can be used for developing cAMP assays.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin-cAMP conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM101.203 µL506.017 µL1.012 mL5.06 mL10.12 mL
5 mM20.241 µL101.203 µL202.407 µL1.012 mL2.024 mL
10 mM10.12 µL50.602 µL101.203 µL506.017 µL1.012 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy5 biotin conjugate65167025000010.271, 0.420.020.03
Cy3 biotin conjugate55556915000010.1510.070.073
Cy7 biotin conjugate7567792500000.30.050.036

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Citations


View all 60 citations: Citation Explorer
cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors
Authors: Liu, Cong and Sun, Di and Liu, Jiawen and Chen, Ying and Zhou, Xuge and Ru, Yunrui and Zhu, Jingrong and Liu, Weijie
Journal: Nature communications (2022): 1--15
Roles of Cyclic AMP Response Element Binding Activation in the ERK1/2 and p38 MAPK Signalling Pathway in Central Nervous System, Cardiovascular System, Osteoclast Differentiation and Mucin and Cytokine Production
Authors: Koga, Y., Tsurumaki, H., Aoki-Saito, H., Sato, M., Yatomi, M., Takehara, K., Hisada, T.
Journal: Int J Mol Sci (2019): ersion="1.0" encoding="UTF-8" ?>17102.enlEndN
Making and Breaking of an Essential Poison: the Cyclases and Phosphodiesterases That Produce and Degrade the Essential Second Messenger Cyclic di-AMP in Bacteria
Authors: Commichau, F. M., Heidemann, J. L., Ficner, R., Stulke, J.
Journal: J Bacteriol (2019): se name="17102.enl" path="C:\Users\aatbi\Drop
Cyclic di-AMP in host-pathogen interactions
Authors: Devaux, L., Kaminski, P. A., Trieu-Cuot, P., Firon, A.
Journal: Curr Opin Microbiol (2018): 21-28
Natural products as modulators of the cyclic-AMP pathway: evaluation and synthesis of lead compounds
Authors: Sengupta, S., Mehta, G.
Journal: Org Biomol Chem (2018): 6372-6390
Perspective of ions and messengers: an intricate link between potassium, glutamate, and cyclic di-AMP
Authors: Gundlach, J., Commichau, F. M., Stulke, J.
Journal: Curr Genet (2018): 191-195
Regulation of IP3 receptors by cyclic AMP
Authors: Taylor, C. W.
Journal: Cell Calcium (2017): 48-52
The cyclic AMP phosphodiesterase 4D5 (PDE4D5)/receptor for activated C-kinase 1 (RACK1) signalling complex as a sensor of the extracellular nano-environment
Authors: Yarwood, S. J., Parnell, E., Bird, R. J.
Journal: Cell Signal (2017): 282-289
Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA
Authors: Kato, K., Omura, H., Ishitani, R., Nureki, O.
Journal: Annu Rev Biochem (2017): 541-566
Role of cyclic AMP in the eye with glaucoma
Authors: Shim, M. S., Kim, K. Y., Ju, W. K.
Journal: BMB Rep (2017): 60-70