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Biotin-cAMP conjugate
Cyclic adenosine monophosphate (cAMP) is a second messenger used for intracellular signal induction. It is synthesized from adenosine triphosphate (ATP) by enzymes (g-proteins) that are attached to metabotropic receptors and become released when the receptor is activated. Cyclic AMP is involved in the regulation of glycogen, sugar, and lipid metabolism. This cAMP-biotin conjugate is an excellent probe that can be used for developing cAMP assays.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin-cAMP conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 101.203 µL | 506.017 µL | 1.012 mL | 5.06 mL | 10.12 mL |
| 5 mM | 20.241 µL | 101.203 µL | 202.407 µL | 1.012 mL | 2.024 mL |
| 10 mM | 10.12 µL | 50.602 µL | 101.203 µL | 506.017 µL | 1.012 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| APC-Biotin Conjugate | 651 | 660 | 730000 | 0.195 |
| Cy5 biotin conjugate | 651 | 670 | 2500001 | 0.03 |
| PE-Biotin Conjugate | 565 | 574 | 1960000 | 0.175 |
Citations
View all 61 citations: Citation Explorer
2′ 3′-cGAMP interactome identifies 2′ 3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
Authors: Deng, Yu and Hahn, Quentin and Yu, Le and Zhu, Zhichuan and Boyer, Joshua A and Wang, Jian and Kong, Deyu and Carey, Leiah M and Hepperla, Austin J and Simon, Jeremy M and others,
Journal: Science Advances (2024): eado7024
Authors: Deng, Yu and Hahn, Quentin and Yu, Le and Zhu, Zhichuan and Boyer, Joshua A and Wang, Jian and Kong, Deyu and Carey, Leiah M and Hepperla, Austin J and Simon, Jeremy M and others,
Journal: Science Advances (2024): eado7024
cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors
Authors: Liu, Cong and Sun, Di and Liu, Jiawen and Chen, Ying and Zhou, Xuge and Ru, Yunrui and Zhu, Jingrong and Liu, Weijie
Journal: Nature communications (2022): 1--15
Authors: Liu, Cong and Sun, Di and Liu, Jiawen and Chen, Ying and Zhou, Xuge and Ru, Yunrui and Zhu, Jingrong and Liu, Weijie
Journal: Nature communications (2022): 1--15
Roles of Cyclic AMP Response Element Binding Activation in the ERK1/2 and p38 MAPK Signalling Pathway in Central Nervous System, Cardiovascular System, Osteoclast Differentiation and Mucin and Cytokine Production
Authors: Koga, Y., Tsurumaki, H., Aoki-Saito, H., Sato, M., Yatomi, M., Takehara, K., Hisada, T.
Journal: Int J Mol Sci (2019): ersion="1.0" encoding="UTF-8" ?>17102.enlEndN
Authors: Koga, Y., Tsurumaki, H., Aoki-Saito, H., Sato, M., Yatomi, M., Takehara, K., Hisada, T.
Journal: Int J Mol Sci (2019): ersion="1.0" encoding="UTF-8" ?>17102.enlEndN
Making and Breaking of an Essential Poison: the Cyclases and Phosphodiesterases That Produce and Degrade the Essential Second Messenger Cyclic di-AMP in Bacteria
Authors: Commichau, F. M., Heidemann, J. L., Ficner, R., Stulke, J.
Journal: J Bacteriol (2019): se name="17102.enl" path="C:\Users\aatbi\Drop
Authors: Commichau, F. M., Heidemann, J. L., Ficner, R., Stulke, J.
Journal: J Bacteriol (2019): se name="17102.enl" path="C:\Users\aatbi\Drop
Cyclic di-AMP in host-pathogen interactions
Authors: Devaux, L., Kaminski, P. A., Trieu-Cuot, P., Firon, A.
Journal: Curr Opin Microbiol (2018): 21-28
Authors: Devaux, L., Kaminski, P. A., Trieu-Cuot, P., Firon, A.
Journal: Curr Opin Microbiol (2018): 21-28
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