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Biotinylated goat anti-mouse IgG (H+L)

Image comparison of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT's biotinylated goat anti-mouse IgG (H&L) (Left, Cat# 16729) or Jackson's biotinylated goat anti-mouse IgG conjugate (Right). At the end, cells were incubated with AAT's iFluor 488-streptavidin conjugate (Cat# 16955).
Image comparison of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT's biotinylated goat anti-mouse IgG (H&L) (Left, Cat# 16729) or Jackson's biotinylated goat anti-mouse IgG conjugate (Right). At the end, cells were incubated with AAT's iFluor 488-streptavidin conjugate (Cat# 16955).
Ordering information
Price ()
Catalog Number16729
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight150000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
This biotinylated goat anti-mouse IgG (H+L) is affinity-purified to have high affinity. It recognizes both heavy and light chains (H+L). It is biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. It can be used for tissue and cell staining, in situ hybridization, Elispot ELISAs and blots.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biotinylated goat anti-mouse IgG (H+L) to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM666.667 nL3.333 µL6.667 µL33.333 µL66.667 µL
5 mM133.333 nL666.667 nL1.333 µL6.667 µL13.333 µL
10 mM66.667 nL333.333 nL666.667 nL3.333 µL6.667 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
FITC goat anti-mouse IgG (H+L)491516730000.920.254-
Cy3® goat anti-mouse IgG (H+L)55556915000010.1510.0730.07
Cy5® goat anti-mouse IgG (H+L)65167025000010.271, 0.420.030.02
Cy7® goat anti-mouse IgG (H+L)7567792500000.30.0360.05
FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*491516730000.920.254-
Cy3® goat anti-mouse IgG (H+L) *Cross Adsorbed*55556915000010.1510.0730.07
Cy5® goat anti-mouse IgG (H+L) *Cross Adsorbed*65167025000010.271, 0.420.030.02
Cy7® goat anti-mouse IgG (H+L) *Cross Adsorbed*7567792500000.30.0360.05
Biotinylated goat anti-rabbit IgG (H+L)------
Goat anti-mouse IgG (H&L) Agarose------
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.190.25
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.110.3
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.560.43
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Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
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Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures
Authors: Lasne F., undefined
Journal: Methods Mol Biol (2009): 213
Parkinsonism/dystonia syndrome secondary to multiple sclerosis with anti-basal-ganglia antibodies
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Journal: Mov Disord (2009): 309
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