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Cy5® goat anti-mouse IgG (H+L)
AAT Bioquest's anti-mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Cy5-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy5 goat anti-mouse IgG antibodies from other commercial sources, thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT&rsquo;s Cy5<sup>&reg;</sup> goat anti-mouse IgG conjugate (Red, Left) or Jackson&rsquo;s Cy5<sup>&reg;</sup> goat anti-mouse IgG conjugate (Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT&rsquo;s Cy5<sup>&reg;</sup> goat anti-mouse IgG conjugate (Red, Left) or Jackson&rsquo;s Cy5<sup>&reg;</sup> goat anti-mouse IgG conjugate (Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
CatalogSize
Price
Quantity
168551 mg
Price
 
Physical properties

Molecular weight~150000
SolventWater
Spectral properties

Correction factor (260 nm)0.02
Correction factor (280 nm)0.03
Correction factor (482 nm)0.009
Correction factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)651
Emission (nm)670
Quantum yield
0.27
1
0.4
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on September 21, 2025