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Biotin PEG 4 Alkyne

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Physical properties
Molecular weight281.37
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Biotin and biotin derivatives bind with high affinity to both avidins and streptavidins. Biotin alkyne can be readily conjugated to many biomolecules through the well-known click chemistry.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin PEG 4 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM355.404 µL1.777 mL3.554 mL17.77 mL35.54 mL
5 mM71.081 µL355.404 µL710.808 µL3.554 mL7.108 mL
10 mM35.54 µL177.702 µL355.404 µL1.777 mL3.554 mL

Molarity calculator

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Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



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Ultrasensitive microfluidic immunosensor with stir bar enrichment for point-of-care test of Staphylococcus aureus in foods triggered by DNAzyme-assisted click reaction.
Authors: Wang, Jiaqi and Tan, Lei and Bi, Wenchao and Shen, Haoyu and Li, Dengfeng and Yu, Zhenzhong and Gan, Ning
Journal: Food chemistry (2022): 132093
Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.
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Journal: Biotechnology and bioengineering (2021): 1520-1530
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Journal: Nature methods (2021): 107-113
Click and Release Chemistry for Activity-Based Purification of β-Lactam Targets.
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Liposome Click Membrane Permeability Assay for Identifying Permeable Peptides.
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