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Biotin PEG 4 Alkyne

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Physical properties
Molecular weight281.37
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


CAS
1006592-45-5
Molecular weight
281.37
Biotin and biotin derivatives bind with high affinity to both avidins and streptavidins. Biotin alkyne can be readily conjugated to many biomolecules through the well-known click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin PEG 4 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM355.404 µL1.777 mL3.554 mL17.77 mL35.54 mL
5 mM71.081 µL355.404 µL710.808 µL3.554 mL7.108 mL
10 mM35.54 µL177.702 µL355.404 µL1.777 mL3.554 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 50 references: Citation Explorer
Ultrasensitive microfluidic immunosensor with stir bar enrichment for point-of-care test of Staphylococcus aureus in foods triggered by DNAzyme-assisted click reaction.
Authors: Wang, Jiaqi and Tan, Lei and Bi, Wenchao and Shen, Haoyu and Li, Dengfeng and Yu, Zhenzhong and Gan, Ning
Journal: Food chemistry (2022): 132093
Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.
Authors: Calle, Beatriz and Bineva-Todd, Ganka and Marchesi, Andrea and Flynn, Helen and Ghirardello, Mattia and Tastan, Omur Y and Roustan, Chloe and Choi, Junwon and Galan, M Carmen and Schumann, Benjamin and Malaker, Stacy A
Journal: Journal of the American Society for Mass Spectrometry (2021): 2366-2375
A highly sensitive and dual-readout immunoassay for norfloxacin in milk based on QDs-FM@ALP-SA and click chemistry.
Authors: Liu, Xiao and Xu, Zehua and Han, Zhenyu and Fan, Longxing and Liu, Sha and Yang, Han and Chen, Zongfen and Sun, Tieqiang and Ning, Baoan
Journal: Talanta (2021): 122703
Profiling sirtuin activity using Copper-free click chemistry.
Authors: Curry, Alyson M and Cohen, Ian and Zheng, Song and Wohlfahrt, Jessica and White, Dawanna S and Donu, Dickson and Cen, Yana
Journal: Bioorganic chemistry (2021): 105413
A peptide-based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging.
Authors: Nöth, Maximilian and Zou, Zhi and El-Awaad, Islam and de Lencastre Novaes, Letícia C and Dilarri, Guilherme and Davari, Mehdi D and Ferreira, Henrique and Jakob, Felix and Schwaneberg, Ulrich
Journal: Biotechnology and bioengineering (2021): 1520-1530
Plasmon-assisted click chemistry at low temperature: an inverse temperature effect on the reaction rate.
Authors: Guselnikova, Olga and Váňa, Jiří and Phuong, Linh Trinh and Panov, Illia and Rulíšek, Lubomír and Trelin, Andrii and Postnikov, Pavel and Švorčík, Václav and Andris, Erik and Lyutakov, Oleksiy
Journal: Chemical science (2021): 5591-5598
Click-ExM enables expansion microscopy for all biomolecules.
Authors: Sun, De-En and Fan, Xinqi and Shi, Yujie and Zhang, Hao and Huang, Zhimin and Cheng, Bo and Tang, Qi and Li, Wei and Zhu, Yuntao and Bai, Jinyi and Liu, Wei and Li, Yuan and Wang, Xiaoting and Lei, Xiaoguang and Chen, Xing
Journal: Nature methods (2021): 107-113
Click and Release Chemistry for Activity-Based Purification of β-Lactam Targets.
Authors: Saidjalolov, Saidbakhrom and Braud, Emmanuelle and Edoo, Zainab and Iannazzo, Laura and Rusconi, Filippo and Riomet, Margaux and Sallustrau, Antoine and Taran, Frédéric and Arthur, Michel and Fonvielle, Matthieu and Etheve-Quelquejeu, Mélanie
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2021): 7687-7695
Liposome Click Membrane Permeability Assay for Identifying Permeable Peptides.
Authors: Desai, Tanvi J and Habulihaz, Bahanu and Cannon, Joe R and Chandramohan, Arun and Kaan, Hung Yi Kristal and Sadruddin, Ahmad and Yuen, Tsz Ying and Johannes, Charles and Thean, Dawn and Brown, Chris J and Lane, David P and Partridge, Anthony W and Evers, Raymond and Sawyer, Tomi K and Hochman, Jerome
Journal: Pharmaceutical research (2021): 843-850
Biofunctional Polymer Coated Au Nanoparticles Prepared via RAFT-Assisted Encapsulating Emulsion Polymerization and Click Chemistry.
Authors: Pereira, Sónia O and Trindade, Tito and Barros-Timmons, Ana
Journal: Polymers (2020)