AAT Bioquest

AssayWise Letters 2013, Vol. 2(1)

Non-Radioactive Glucose Uptake Assays
Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring the uptake of 2 deoxyglucose (2-DG), a glucose analog, is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and

Multicolor Live or Dead™ Viability Assays for Flow Cytometry
Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools to labeling cells for fluorescence microscopic and flow cytometric investigations of cellular functions. AAT Bioquest offers a set of multicolor cell labeling kits to uniformly label dead mammalian cells for long term microscopic examination

Colorimetric Quantitation of Ammonia
Elevated levels of ammonia in blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hyperammonemia is associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). The determination of ammonia is very useful for clinical laboratory to monitor

Fluorimetric Quantitation of Formaldehyde
Formaldehyde is the primary cause of methanol's toxicity, since methanol is metabolized into toxic formaldehyde by alcohol dehydrogenase. AAT Bioquest's Amplite™ Fluorimetric Formaldehyde Quantitation Kit is used for quantifying formaldehyde. The kit uses a proprietary fluorogenic dye that

Fluorimetric Quantitation of Lactate Dehydrogenase
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyzes the interconversion of pyruvate and lactate.Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage

Fluorimetric Measurement of Glucose-6-Phosphate
Glucose-6-phosphate (G6P) is a key intermediate utilized by glucose-6-phosphate dehydrogenase (G6PD) to generate the reducing equivalents in the form of NADPH. This is particularly important in red blood cells where G6PD deficiency leads to hemolytic anemia. Amplite™ Fluorimetric Glucose-6-Phosphate

Detection of Reactive Oxygen Species (ROS) Using Multicolor ROS Brite™ Reagents
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen (such as superoxide, hydroxyl radical, singlet oxygen and peroxides). ROS forms as a natural byproduct of the normal metabolism of oxygen and plays important roles in cell signaling and homeostasis. Increased levels

Fluo-8® Green Calcium Indicators The Brightest Ca2+ Signal at 488 nm Excitation
Fluo-3 and Fluo-4 were the most commonly used visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes

Reporter Gene Analysis Gaussia Luciferase Reporter Gene Assay
For experiments which require smaller molecules and are not dependent on the presence of ATP, gaussia luciferase is a useful reporter gene to demonstrate expression in mammalian cells. Using a proprietary luminogenic formulation, the AmpliteTM Gaussia Luciferase Reporter Gene Assay kit has a consistent,

Reporter Gene Analysis Firefly Luciferase Reporter Gene Assay
The bioluminescent protein firefly luciferase is almost universally applicable for experiments, due to its lack of toxicity and use in both pro-and eukaryotic cells. Commonly used as a reporter gene, AAT Bioquest offers a proprietary DTT-free formula in the all-inclusive AmpliteTM Luciferase

iFluor™ 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, DyLight™ 594 and Alexa Fluor® 594 Dyes
Displaying excellent brightness and compatibility with common instrumentation, the iFluorTM 594 dye family is a useful improvement for other red dyes such as Alexa Fluor® or Texas Red®. The color allows for minimal overlap with other common green-fluorescent dyes, allowing multicolor readings

trFluor™ Fluorescent Labeling Dyes and Their Bioconjugates *Optimized for TR-FRET Applications*
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules

ReadiLink™ iFluor™ Antibody Labeling Kits
ReadiLink™ iFluor™ Antibody Labeling Kits provide a convenient way to label antibodies using a stable reactive form of the iFluor™ dyes. ReadiLink™ Kits only require two simple mixing steps to produce the desired conjugates for flow cytometry and fluorescence imaging applications. The conjugation