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ReadiLink™ iFluor® Antibody Labeling Kits
ReadiLink™ iFluor® Antibody Labeling Kits provide a convenient way to label antibodies using a stable reactive form of the iFluor® dyes. The reactive iFluor® dyes show good reactivity and selectivity with the aliphatic amines of antibodies and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond. iFluor®-antibody conjugates may be used for immunofluorescent staining, fluorescent in situ hybridization, flow cytometry and other biological applications. Each kit comes with all the essential components for performing the conjugation reaction and for purifying the iFluor®-antibody conjugates. ReadiLink™ Kits only require two simple mixing steps to produce the desired conjugates for flow cytometry and fluorescence imaging applications. The conjugation kits provide the best method for readily labeling small amount of antibodies without requiring column purification.
Key Features of ReadiLink™ Kits :
- Complete, all the components provided in the kits.
- Robust, only two simple mixing steps required.
- Rapid, less than 10 minutes hands-on time.
Fig. 1
ReadiLink™ Kit Labeling Principle
- Start the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the Reaction Buffer (pH 7.5-8.5).
- Incubate the reaction solution and get a mixture of the desired protein conjugate and unreactive free dye.
- Quench the reaction by mixing a non-fluorescent Tide Quencher™ (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-labeling dye complex, which eliminates the background fluorescence interference of the free labeling dye.
Document: 03.0039.130501r1
Last updated Thu Oct 02 2025
ReadiLink™ iFluor® Antibody Labeling Kits