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AAT Bioquest

ReadiLink™ iFluor™ Antibody Labeling Kits

ReadiLink™ iFluor® Antibody Labeling Kits provide a convenient way to label antibodies using a stable reactive form of the iFluor® dyes. The reactive iFluor® dyes show good reactivity and selectivity with the aliphatic amines of antibodies and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond. iFluor®-antibody conjugates may be used for immunofluorescent staining, fluorescent in situ hybridization, flow cytometry and other biological applications. Each kit comes with all the essential components for performing the conjugation reaction and for purifying the iFluor®-antibody conjugates. ReadiLink™ Kits only require two simple mixing steps to produce the desired conjugates for flow cytometry and fluorescence imaging applications. The conjugation kits provide the best method for readily labeling small amount of antibodies without requiring column purification.

Key Features of ReadiLink™ Kits :

  • Complete, all the components provided in the kits.
  • Robust, only two simple mixing steps required.
  • Rapid, less than 10 minutes hands-on time.

Readilink kit labeling principle
Readilink kit labeling principle key

ReadiLink™ Kit Labeling Principle
  1. Start the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the Reaction Buffer (pH 7.5-8.5).
  2. Incubate the reaction solution and get a mixture of the desired protein conjugate and unreactive free dye.
  3. Quench the reaction by mixing a non-fluorescent Tide Quencher™ (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-labeling dye complex, which eliminates the background fluorescence interference of the free labeling dye.

 

Table 1. ReadiLink™ Rapid iFluor® Antibody Labeling Kits

ReadiLink™ Antibody Labeling Kit
Fluorophore Equivalent
Ex/Em (nm)
Imaging
Flow Cytometry
Cat No.
ReadiLink™ Rapid iFluor® 350AMCA, Alexa Fluor® 350344/448Yes 1220
ReadiLink™ xtra Rapid iFluor® 350AMCA, Alexa Fluor® 350344/448Yes 1950
ReadiLink™ Rapid iFluor® 488FITC, Alexa Fluor® 488491/516YesYes1255
ReadiLink™ xtra Rapid iFluor® 488FITC, Alexa Fluor® 488491/516YesYes1955
ReadiLink™ Rapid iFluor® 555Cy3, Alexa Fluor® 555556/569Yes 1227
ReadiLink™ xtra Rapid iFluor® 555Cy3, Alexa Fluor® 555556/569Yes 1958
ReadiLink™ Rapid iFluor® 594Texas Red®, Alexa Fluor® 594587/603Yes 1230
ReadiLink™ xtra Rapid iFluor® 594Texas Red®, Alexa Fluor® 594587/603Yes 1960
ReadiLink™ Rapid iFluor® 633Cy5, Alexa Fluor® 633638/655YesYes1260
ReadiLink™ Rapid iFluor® 647Cy5, Alexa Fluor® 647654/669YesYes1235
ReadiLink™ xtra Rapid iFluor® 647Cy5, Alexa Fluor® 647654/669YesYes1963
ReadiLink™ Rapid iFluor® 680Cy5.5, Alexa Fluor® 680, IRDye® 700682/701YesYes1240
ReadiLink™ Rapid iFluor® 700Alexa Fluor® 700693/713YesYes1245
ReadiLink™ Rapid iFluor® 750Cy7, Alexa Fluor® 750759/777Yes 1250
ReadiLink™ xtra Rapid iFluor® 750Cy7, Alexa Fluor® 750759/777Yes 1965
ReadiLink™ Rapid iFluor® 790IRDye® 800, Alexa Fluor® 790782/811YesYes1265