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iFluor® 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, DyLight™ 594 and Alexa Fluor® 594 Dyes
iFluor® 594 has spectral characteristics similar to those of Texas Red®, DyLight™ 594 and Alexa Fluor® 594 with excitation and emission wavelength at ~592/614 nm when conjugated to proteins. iFluor® 594 dye has superior labeling performance and better stability than Texas Red®. Our iFluor® 594 conjugated streptavidin provides high fluorescence intensity and low background. Biomolecules conjugated to iFluor® 594 exhibit little spectral overlap with green-fluorescent conjugates and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. The minimal spectral overlap makes iFluor® 594 an ideal second color in combination with a green color such as GFP, FITC, Alexa Fluor® 488 or iFluor® 488. Our in-house research indicated that the iFluor® 594-RPE conjugates demonstrate better FRET than Alexa Fluor® 594-RPE.
Fig. 1
The excitation and emission spectra of iFluor® 594 Goat Anti-Rabbit IgG Conjugate in PBS buffer (pH 7.2)
Fig. 2
Left: HeLa cells were stained with Rabbit HDAC antibody, and followed with iFluor® 594 Goat anti-rabbit IgG Conjugate and Alexa Fluor® 594 goat anti-rabbit IgG Conjugate respectively under the same conditions. The iFluorTM 594 Goat Anti-Rabbit IgG conjugate (left panel) demonstrated much lower staining background than the corresponding Alexa Fluor® 594 (right panel). Right: HeLa cells were stained with mouse anti-tubulin followed with iFluor® 594 Goat Anti-Mouse IgG, and nuclei were stained with Hoechst 33342.
Document: 03.0037.130501r1
Last updated Fri Oct 03 2025
iFluor® 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, DyLight™ 594 and Alexa Fluor® 594 Dyes