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iFluor™ 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, DyLight™ 594 and Alexa Fluor® 594 Dyes

iFluor 594 Goat Anti-rabbit IgG
The excitation and emission spectra of iFluor® 594 Goat Anti-Rabbit IgG Conjugate in PBS buffer (pH 7.2)
iFluor® 594 has spectral characteristics similar to those of Texas Red®, DyLight™ 594 and Alexa Fluor® 594 with excitation and emission wavelength at ~592/614 nm when conjugated to proteins. iFluor® 594 dye has superior labeling performance and better stability than Texas Red®. Our iFluor® 594 conjugated streptavidin provides high fluorescence intensity and low background. Biomolecules conjugated to iFluor® 594 exhibit little spectral overlap with green-fluorescent conjugates and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. The minimal spectral overlap makes iFluor® 594 an ideal second color in combination with a green color such as GFP, FITC, Alexa Fluor® 488 or iFluor® 488. Our in-house research indicated that the iFluor® 594-RPE conjugates demonstrate better FRET than Alexa Fluor® 594-RPE.

HeLa cells were stained with Rabbit HDAC antibody.
HeLa cells were stained with mouse anti-tubulin.

Left: HeLa cells were stained with Rabbit HDAC antibody, and followed with iFluor® 594 Goat anti-rabbit IgG Conjugate and Alexa Fluor® 594 goat anti-rabbit IgG Conjugate respectively under the same conditions. The iFluorTM 594 Goat Anti-Rabbit IgG conjugate (left panel) demonstrated much lower staining background than the corresponding Alexa Fluor® 594 (right panel). Right: HeLa cells were stained with mouse anti-tubulin followed with iFluor® 594 Goat Anti-Mouse IgG, and nuclei were stained with Hoechst 33342.

 

Table 1. iFluor® 594 specifications

Product Name
Ex (nm)
Em (nm)
Ext. Coeff. (cm-1M-1)
CF280 nm*
CF260 nm**
iFluor® 594586611120,0000.06050.0945
  1. *CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm.
  2. **CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).

Table 2. Product Ordering Information for iFluor® 594

Cat No.
Product Name
Ex (nm)
Em (nm)
Unit Size
20073Annexin V-iFluor® 594 conjugate 590615100 Tests
16468Annexin V-iFluor® 594 conjugate 592614200 ug
16628iFluor® 594 goat anti-rabbit IgG (H+L) 592614200 ug
2577ReadiUse™ Preactivated PE-iFluor® 647 Tandem 5656741 mg
16962iFluor® 594-streptavidin conjugate 592614200 ug
1029iFluor® 594 succinimidyl ester 5946141 mg
1230ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* 5926142 Labelings