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iFluor® 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, Dylight™ 594 and Alexa Fluor® 594 Dyes
iFluor® 594 has spectral characteristics similar to those of Texas Red®, DyLight™ 594 and Alexa Fluor® 594 with excitation and emission wavelength at ~592/614 nm when conjugated to proteins. iFluor® 594 dye has superior labeling performance and better stability than Texas Red®. Our iFluor® 594 conjugated streptavidin provides high fluorescence intensity and low background. Biomolecules conjugated to iFluor® 594 exhibit little spectral overlap with green-fluorescent conjugates and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. The minimal spectral overlap makes iFluor® 594 an ideal second color in combination with a green color such as GFP, FITC, Alexa Fluor® 488 or iFluor® 488. Our in-house research indicated that the iFluor® 594-RPE conjugates demonstrate better FRET than Alexa Fluor® 594-RPE.
Fig. 1
The excitation and emission spectra of iFluor® 594 Goat Anti-Rabbit IgG Conjugate in PBS buffer (pH 7.2)
Fig. 2
Left: HeLa cells were stained with Rabbit HDAC antibody, and followed with iFluor® 594 Goat anti-rabbit IgG Conjugate and Alexa Fluor® 594 goat anti-rabbit IgG Conjugate respectively under the same conditions. The iFluorTM 594 Goat Anti-Rabbit IgG conjugate (left panel) demonstrated much lower staining background than the corresponding Alexa Fluor® 594 (right panel). Right: HeLa cells were stained with mouse anti-tubulin followed with iFluor® 594 Goat Anti-Mouse IgG, and nuclei were stained with Hoechst 33342.
This document (03.0037.130501r1) was last updated on Wed Oct 29 2025. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
iFluor® 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, Dylight™ 594 and Alexa Fluor® 594 Dyes