Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Aggrecan

Aggrecan, a proteoglycan, is a key component of cartilage structure and the function of joints. It provides articular cartilage with structural support and load-bearing properties through facilitated hyaluronan interactions that form stable ternary complexes in the extracellular matrix. Crucial to chondro-skeletal morphogenesis during fetal development, the structural motif of aggrecan is similar to that of a link protein, as it is composed of three globular domains - G1, G2, and G3 - and a large extended region to allow for glycosaminoglycan attachment. The three globular domains are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Faults in functional properties of aggrecan are involved with many cellular pathways involving degenerative joint and bone diseases, including osteoarthritis, spinal, cranial, and epi-/metaphyseal abnormalities.

Aggrecan has been identified by Western blot and similar electrophoretic techniques and analyzed with RT-PCR. Related assays include those associated with hyaluronan binding or immunostaining to assess collagen potential.
 

Table 1. Aggrecan at-a-glance.

Marker
Synonyms
Mol Wt.
Cell Types
Database Links
AggrecanACAN
Cartilage-Specific Proteoglycan Core Protein
Chondroitin Sulfate Proteoglycan 1
CSPCP
CSPG1
PGCA
>2,500 kDaChondrocytesGene ID: 176
Swiss-Port: P16112
OMIM: 155760

Table 2. Aggrecan Antibodies.

Product
Clonality
Host
Reactivity
Conjugate
Application Dilutions
Unit Size
Cat No.
Aggrecan (Cleaved-Asp369) AntibodyPolyclonalRabbitHuman, RatUnconjugatedWB: 1:500∼1:1000
IHC: 1:50∼1:100
ELISA: 1:20000
50 µg8L0101

References


  1. Kiani, C., Chen, L., Wu, Y. J., Yee, A. J., & Yang, B. B. (2002). Structure and function of aggrecan. Cell research, 12(1), 19-32. https://doi.org/10.1038/sj.cr.7290106


Original created on March 7, 2022, last updated on March 7, 2022
Tagged under: Aggrecan, ACAN, Cartilage-Specific Proteoglycan Core Protein, Chondroitin Sulfate Proteoglycan 1, CSPCP, CSPG1, PGCA