Crosslinking is a technique that chemically joins two or more proteins together, such as an antibody and an enzyme, by a covalent bond. Crosslinking reagents consist of reactive moieties that modify and attach to specific functional groups on proteins. A common approach for conjugating two biomolecules utilizes a small, heterobifunctional crosslinker called succinimidyl 4-(N-maleimidomethyl) cyclohexane1-carboxylate (SMCC). SMCC crosslinkers contain amine-reactive NHS ester moieties and thiol-reactive maleimide moieties to crosslink proteins. However, a drawback to this approach is the many experimental conditions that must be monitored to perform a successful conjugation. Difficulties associated with SMCC conjugations are maintenance of a narrow pH range, self-polymerization, and hydrolytic degradation of reactive moieties. To address these concerns, AAT Bioquest has developed an optimized and robust Buccutite™ crosslinking technique. Buccutite™ crosslinking technology provides a simplistic and efficient approach for conjugating proteins. It can be completed in half the time and with less stringent parameters yielding highly stable and easy-to-handle conjugated proteins.

Buccutite™ conjugation can be run in a broad pH range of 5-9 and temperature.

AAT Bioquest offers same-day custom conjugation of proteins or antibodies with a wide array of labels, such as biotin, HRP and over 20 fluorophores. Our services are designed to produce high-quality results with a fast turnaround. We take pride in supplying scientists with flexible tools that help expand their research needs.

Note: APC and PE custom conjugation services require 3 working days You may supply your own protein or antibody or chose from 3000+ monoclonal and polyclonal antibodies in our catalog. Contact us for a quote today, and let us put our expertise to work for you!