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Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications

Tide Fluor™ Dyes for Fluorescence Labeling and FRET Applications


  • Optimized to label peptides and nucleotides with performance comparable to Alexa Fluor® Dyes
  • Optimized to pair with Tide Quencher™ dark acceptors to maximize FRET efficiency
  • pH-insensitive and environment-insensitive fluorescence for developing robust assays
  • Higher photostability to improve the quality of fluorescence imaging
  • A variety of reactive forms available for conjugations
The sulfonated cyanine dyes have improved fluorescence quantum yield than those of non-sulfonate cyanines. They are commercially available as Cy3®, Cy5®, Cy5.5® and Cy7®. However, these commercial protein-labeling CyDyes and Alexa Fluor® dyes are cost-prohibitive for labeling peptides and oligonucleotides that require much larger amount of labeling dyes. The improved and affordable Tide Fluor™ dyes are optimized as building blocks for developing FRET oligonucleotides and peptides. Our Tide Fluor™ dyes have stronger fluorescence and higher photostability than the classic fluorophores (such as fluoresceins, rhodamines and cyanines). They are the best affordable fluorescent dyes for labeling peptides and oligonucleotides without comprised performance.
 

Table 1. Tide Fluor™ Dyes for Developing FRET Probes

Tide Fluor™ Donor
Ex (nm)
Em (nm)
Features and Benefits
Ordering Information
Tide Fluor™ 1 (TF1)345442
  • Alternative to EDANS
  • Much stronger absorption
  • Much stronger fluorescence
  • Less environmental sensitivity
Cat# 2236 (TF1 azide, click chemistry)
Cat# 2237 (TF1 alkyne, click chemistry)
Cat# 2238 (TF1 acid)
Cat# 2239 (TF1 amine)
Cat# 2242 (TF1 maleimide, SH-reactive)
Cat# 2244 (TF1 SE, NH2-reactive)
Tide Fluor™ 2 (TF2)500527
  • Alternative to FAM, FITC and Alexa Fluor® 488
  • pH-insensitive fluorescence
  • Good photostability
Cat# 2245 (TF1 acid)
Cat# 2246 (TF2 amine)
Cat# 2247 (TF2 maleimide SH-reactive)
Cat# 2248 (TF2 SE, NH2-reactive)
Cat# 2252 (TF2 azide, click chemistry)
Cat# 2253 (TF2 alkyne, click chemistry)
Tide Fluor™ 3 (TF3)555584
  • Alternative to Cy3® and Alexa Fluor® 555
  • Strong fluorescence
  • Good photostability
Cat# 2254 (TF3 azide, click chemistry)
Cat# 2255 (TF3 alkyne, click chemistry)
Cat# 2268 (TF3 acid)
Cat# 2269 (TF3 amine)
Cat# 2270 (TF3 maleimide, SH-reactive)
Cat# 2271 (TF3 SE, NH2-reactive)
Tide Fluor™ 3WS (TF3WS)555565
  • Alternative to Cy3® and Alexa Fluor® 555
  • Strong fluorescence
  • Good photostability
Cat# 2345 (TF3WS acid)
Cat# 2346 (TF3WS SE, NH2-reactive)
Tide Fluor™ 4 (TF4)590618
  • Alternative to ROX, Texas Red® and Alexa Fluor® 594
  • Strong fluorescence
  • Good photostability
Cat# 2285 (TF4 acid)
Cat# 2286 (TF4 amine)
Cat# 2287 (TF4 maleimide, SH-reactive)
Cat# 2289 (TF4 SE, NH2-reactive)
Cat# 2300 (TF4 azide, click chemistry)
Cat# 2301 (TF4 alkyne, click chemistry)
Tide Fluor™ 5WS (TF5WS)649664
  • Alternative to Cy5® and Alexa Fluor® 647
  • Strong fluorescence
  • Good photostability
Cat# 2275 (TF5WS azide, click chemistry)
Cat# 2276 (TF5WS alkyne, click chemistry)
Cat# 2278 (TF5WS, acid)
Cat# 2279 (TF5WS amine)
Cat# 2280 (TF5WS maleimide, SH-reactive)
Cat# 2281 (TF5WS SE, NH2-reactive)
Tide Fluor™ 6WS (TF6WS)676695
  • Alternative to Cy5.5®, IRDye® 700 and Alexa Fluor® 680
  • Strong fluorescence
  • Good photostability
Cat# 2291 (TF6WS acid)
Cat# 2292 (TF6WS amine)
Cat# 2293 (TF6WS maleimide, SH-reactive)
Cat# 2294 (TF6WS SE, NH2-reactive)
Cat# 2302 (TF6WS azide, click chemistry)
Cat# 2303 (TF6WS alkyne, click chemistry)
Tide Fluor™ 7WS (TF7WS)749775
  • Alternative to Cy7® and Alexa Fluor® 750
  • Strong fluorescence
  • Good photostability
Cat# 2304 (TF7WS azide, click chemistry)
Cat# 2305 (TF7WS alkyne, click chemistry)
Cat# 2330 (TF7WS acid)
Cat# 2331 (TF7WS amine)
Cat# 2332 (TF7WS maleimide, SH-reactive)
Cat# 2333 (TF7WS SE, NH2-reactive)
Tide Fluor™ 8WS (TF8WS)775807
  • Alternative to IRDye® 800
  • Strong fluorescence
  • Good photostability
Cat# 2306 (TF8WS azide, click chemistry)
Cat# 2307 (TF8WS alkyne, click chemistry)
Cat# 2335 (TF8WS acid)
Cat# 2336 (TF8WS amine)
Cat# 2337 (TF8WS maleimide, SH-reactive)
Cat# 2338 (TF8WS SE, NH2-reactive)

 

Chemical Properties of Tide Fluor™ Fluorescent Labeling Dyes


Tide Fluor™ dyes are a series of excellent fluorescent labeling dyes that span the full visible spectrum. Tide Fluor™ dyes have improved labeling performance than the classic fluorescent labeling dyes such as FITC, TRITC, Texas Red®, Cy3, Cy5 and Cy7. They are the best affordable fluorescent dyes (alternative to Alexa Fluor® dyes) for labeling oligos and peptides without comprised performance. Each Tide Fluor™ dye is developed to match the spectral properties of a particular Alexa Fluor® or other labeling dyes (such as DyLight™ dyes).
 

Table 2. Chemical Properties of Tide Fluor™ Fluorescent Labeling Dyes

Cat No.
Labeling Dye
Reactivity
Adduct MW Calculation*
2236Tide Fluor™ 1 azide [TF1 azide]Alkyne+ 301
2237Tide Fluor™ 1 alkyne [TF1 alkyne]Azide+ 270
2238Tide Fluor™ 1 acid [TF1 acid] *Superior replacement for EDANS*NH2 and OH+ 215
2239Tide Fluor™ 1 amine [TF1 amine] *Superior replacement for EDANS*CO2H+ 257
2242Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS*SH+ 355
2244Tide Fluor™ 1 succinimidyl ester [TF1 SE]Aliphatic amine+ 215
2245Tide Fluor™ 2 acid [TF2 acid] *Superior replacement for fluorescein*NH2 and OH+ 469
2246Tide Fluor™ 2 amine [TF2 amine] *Superior replacement for fluorescein*CO2H+ 398
2247Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein*SH+ 680
2248Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein*Aliphatic amine+ 469
2252Tide Fluor™ 2 azide [TF2 azide]Alkyne+ 555
2253Tide Fluor™ 2 alkyne [TF2 alkyne]Azide+ 524
2348Tide Fluor™ 2WS acid [TF2WS acid] *Superior replacement for FITC*NH2 and OH+ 1095
2349Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] *Superior replacement for FITC*Aliphatic amine+ 1095
2254Tide Fluor™ 3 azide [TF3 azide]Alkyne+ 526
2255Tide Fluor™ 3 alkyne [TF3 alkyne]Azide+ 495
2268Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3*NH2 and OH+ 440
2269Tide Fluor™ 3 amine [TF3 amine] *Superior replacement for Cy3*CO2H+ 496
2270Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3*SH+ 580
2271Tide Fluor™ 3 succinimidyl ester [TF3 SE]*Superior replacement for Cy3*Aliphatic amine+ 440
2345Tide Fluor™ 3WS acid [TF3WS acid] *Superior replacement for Cy3*NH2 and OH+ 706
2346Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] *Superior replacement for Cy3*Aliphatic amine+ 706
2285Tide Fluor™ 4 acid [TF4 acid] *Superior replacement for ROX and Texas Red*NH2 and OH+ 544
2286Tide Fluor™ 4 amine [TF4 amine] *Superior replacement for ROX and Texas Red*CO2H+ 586
2287Tide Fluor™ 4 maleimide [TF4 maleimide] *Superior replacement for ROX and Texas Red*SH+ 755
2289Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red*Aliphatic amine+ 544
2300Tide Fluor™ 4 azide [TF4 azide]Alkyne+ 630
2301Tide Fluor™ 4 alkyne [TF4 alkyne]Azide+ 599
2275Tide Fluor™ 5WS azide [TF5WS azide]Alkyne+ 818
2276Tide Fluor™ 5WS alkyne [TF5WS alkyne]Azide+ 787
2278Tide Fluor™ 5WS acid [TF5WS acid] *Superior replacement for Cy5*NH2 and OH+ 732
2279Tide Fluor™ 5WS amine [TF5WS amine] *Superior replacement for Cy5*CO2H+ 774
2280Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5*SH+ 873
2281Tide Fluor™ 5WS succinimidyl ester [TF5WS SE]*Superior replacement for Cy5*Aliphatic amine+ 732
2291Tide Fluor™ 6WS acid [TF6WS acid] *Superior replacement for Cy5.5*NH2 and OH+ 899
2292Tide Fluor™ 6WS amine [TF6WS amine] *Superior replacement for Cy5.5*CO2H+ 941
2293Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5*SH+ 1039
2294Tide Fluor™ 6WS succinimidyl ester [TF6WS SE]*Superior replacement for Cy5.5*Aliphatic amine+ 899
2302Tide Fluor™ 6WS azide [TF6WS azide]Alkyne+ 1079
2303Tide Fluor™ 6WS alkyne [TF6WS alkyne]Azide+ 1048
2304Tide Fluor™ 7WS azide [TF7WS azide]Alkyne+ 845
2305Tide Fluor™ 7WS alkyne [TF7WS alkyne]Azide+ 813
2330Tide Fluor™ 7WS acid [TF7WS acid] *Superior replacement for Cy7*NH2 and OH+ 758
2331Tide Fluor™ 7WS amine [TF7WS amine] *Superior replacement for Cy7*CO2H+ 801
2332Tide Fluor™ 7WS maleimide [TF7WS maleimide] *Superior replacement for Cy7*SH+ 899
2333Tide Fluor™ 7WS, succinimidyl ester [TF7WS SE]*Superior replacement for Cy7*Aliphatic amine+ 758
2306Tide Fluor™ 8WS azide [TF8WS azide] *Near Infrared Emission*Alkyne+ 1011
2307Tide Fluor™ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*Azide+ 980
2335Tide Fluor™ 8WS acid [TF8WS acid] *Near Infrared Emission*NH2 and OH+ 925
2336Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission*CO2H+ 967
2337Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*SH+ 1065
2338Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*Aliphatic amine+ 925
  1. * The molecular weight of the desired conjugate = the molecular weight of the free unlabeled molecule + the value listed in the table.

 

Spectral Properties of Tide Fluor™ Fluorescent Labeling Dyes


 

Table 3. Tide Fluor™ Dyes Spectral Properties

Labeling Dye
Abs (mn)
Em (nm)
ε¹
Φ²
CF at 260 nm³
CF at 280 nm⁴
Tide Fluor™ 1345442200000.950.2460.187
Tide Fluor™ 2WS491516750000.90.2110.091
Tide Fluor™ 2500527750000.90.2880.201
Tide Fluor™ 3WS5555651500000.1050.0790.079
Tide Fluor™ 3555584850000.850.3310.201
Tide Fluor™ 4590618900000.910.4890.436
Tide Fluor™ 5WS6496642500000.250.0230.027
Tide Fluor™ 6WS6766952200000.180.1110.009
Tide Fluor™ 7WS7497752750000.120.0090.049
Tide Fluor™ 8WS7758072500000.080.1030.109
  1. Extinction Coefficient at their maximum absorption wavelength
  2. FQY = fluorescence quantum yield in aqueous buffer (pH 7.2)
  3. CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligo and nucleic acid labeling)
  4. CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptide and protein labeling)
  5. Fluorescence intensity is significantly increased upon coupled to proteins or long peptides.


Original created on January 3, 2020, last updated on January 3, 2020
Tagged under: Fluorescence Resonance Energy Transfer (FRET), Oligonucleotide Labeling, Peptide Labeling