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Tide Quencher™ Dyes, Optimized FRET Acceptors


The normalized absorption spectra of TQ1, 2, 3, 4, 5, 6 and 7.
Although DABCYL has been used to develop a variety of FRET applications, its low quenching efficiency of longer wavelength dyes (such as fluoresceins, rhodamines and cyanines) has limited its use in the development of sensitive fluorogenic FRET probes. Additionally, the absorption spectrum of DABCYL is environment sensitive. AAT Bioquest has developed the robust Tide Quencher™ acceptor dyes for the development of longer wavelength FRET probes. These Tide Quencher™ dark FRET acceptors (such as TQ1, TQ2, TQ3, TQ4, TQ5, TQ6 and TQ7) are optimized to pair with our Tide Fluor™ dyes and the classic fluorophores (such as AMCA, EDANS, FAM, TAMRA, HEX, JOE, TET, ROX, Cy3®, Cy5® and Cy7®). Like our Tide Fluor™ donor dyes, our Tide Quencher™ acceptor dyes are much more cost-effective with comparable or even better performance for your desired biological applications than other similar products on the market.


The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.
Besides the broad applications in the development of Molecular Beacon probes, our Tide Quencher™ dyes have also been used to develop various protease substrates such as HIV protease, MMPs and secretases. In some cases, they have demonstrated greatly improved enzyme performance. This may be partly due to the redshifted absorption spectrum that overlaps better with the emission spectra of fluoresceins, rhodamines and cyanines. Tide Quencher™ dyes are a great choice for you to eliminate the limitations of classic quenchers. As excellent dark quenchers, Tide Quencher™ dyes are individually optimized to pair with all the popular fluorescent dyes such as fluoresceins, rhodamines and cyanines. Our Tide Quencher™ series of nonfluorescent dyes cover the full visible spectrum with unusually high efficiency. Among them, TQ2 has absorption maximum perfectly matching the emission of FAM while TQ3, TQ5 and TQ7 are proven to be the best quenchers for Cy3®, Cy5® and Cy7®.


HIV protease cleavage of Arg-Glu(5-FAM)-Val-Ser-Phe-Asn-Phe-Pro-Gln-Ile-Thr-Lys(TQ2)-Arg. The substrate was incubated with HIV protease. Upon HIV protease cleavage, the fluorescence of 5-FAM was recovered and monitored at Ex/Em = 490 /520 nm.

The Advantages of Tide Quencher™ Dyes

  • TQ dyes enable you to explore the FRET potentials that might be impossible with other quenchers.
  • Versatile reactive forms are convenient for self-constructing your desired FRET biomolecules.
  • Perfectly match your desired fluorescent donors.
  • Competitive price with better performance.


Table 1. Dye Selection Guide for Preparing FRET Oligonucleotides and Peptides.


 

Table 1. Tide Quencher™ Dyes for Developing FRET Probes

Dark FRET Acceptor
λmax (nm)
Features and Benefits
Ordering Information
Tide Quencher™ 1 (TQ1)490
  • Alternative to Dabcyl, QSY® 35 and BHQ®-0
  • Best paired with Tide Fluor™ 1 (TF1)
  • Excellent FRET efficiency with coumarins
Cat# 2188 (TQ1 azide, click chemistry)
Cat# 2189 (TQ1 alkyne, click chemistry)
Cat# 2190 (TQ1 acid)
Cat# 2192 (TQ1 amine)
Cat# 2193 (TQ1 CPG, OH-reactive)
Cat# 2196 (TQ1 maleimide, SH-reactive)
Cat# 2198 (TQ1 phosphoramidite, OH-reactive)
Cat# 2199 (TQ1 SE, NH2-reactive)
Tide Quencher™ 2 (TQ2)515
  • Alternative to BHQ®-1
  • Best paired with Tide Fluor™ 2 (TF2)
  • Better matched with FAM, FITC and Alexa Fluor® 488 than other commercial quenchers
Cat# 2211 (TQ2 azide, click chemistry)
Cat# 2212 (TQ2 alkyne, click chemistry)
Cat# 2200 (TQ2 acid)
Cat# 2202 (TQ2 amine)
Cat# 2203 & 2204 (TQ2 CPG, OH-reactive)
Cat# 2206 (TQ2 maleimide, SH-reactive)
Cat# 2208 (TQ2 phosphoramidite, OH-reactive)
Cat# 2210 (TQ2 SE, NH2-reactive)
Tide Quencher™ 2WS (TQ2WS)515
  • Alternative to BHQ®-1
  • Best paired with Tide Fluor™ 2 (TF2)
  • Better matched with FAM, FITC and Alexa Fluor® 488 than other commercial quenchers
Cat# 2050 (TQ2WS acid)
Cat# 2058 (TQ2WS SE, NH2-reactive)
Tide Quencher™ 3 (TQ3)570
  • Alternative to QSY® 7, QSY® 9 and BHQ®-2
  • Best paired with Tide Fluor™ 3 (TF3)
  • Excellent FRET efficiency with Cy3®, Alexa Fluor® 555 and TAMRA than other commercial quenchers
Cat# 2200 (TQ3 acid)
Cat# 2222 (TQ3 amine)
Cat# 2223 & 2224 (TQ3 CPG, OH-reactive)
Cat# 2226 (TQ3 maleimide, SH-reactive)
Cat# 2228 (TQ3 phosphoramidite, OH-reactive)
Cat# 2230 (TQ3 SE, NH2-reactive)
Cat# 2231 (TQ3 azide, click chemistry)
Cat# 2232 (TQ3 alkyne, click chemistry)
Tide Quencher™ 3WS (TQ3WS)578
  • Alternative to QSY® 7, QSY® 9 and BHQ®-2
  • Best paired with Tide Fluor™ 3 (TF3)
  • Excellent FRET efficiency with Cy3®, Alexa Fluor® 555 and TAMRA than other commercial quenchers
Cat# 2227 (TQ3WS acid)
Cat# 2229 (TQ3WS SE, NH2-reactive)
Tide Quencher™ 4 (TQ4)603
  • Strong absorption
  • Best paired with Tide Fluor™ 4 (TF4)
  • Better FRET efficiency with ROX, Texas Red® and Alexa Fluor® 594 than other commercial quenchers
Cat# 2062 & 2063 (TQ4 CPG, OH-reactive)
Tide Quencher™ 4WS (TQ4WS)~590
  • Strong absorption
  • Best paired with Tide Fluor™ 4 (TF4)
  • Better FRET efficiency with ROX, Texas Red® and Alexa Fluor® 594 than other commercial quenchers
Cat# 2060 (TQ4WS acid)
Cat# 2061 (TQ4WS amine)
Cat# 2064 (TQ4WS maleimide, SH-reactive)
Cat# 2067 (TQ4WS SE, NH2-reactive)
Cat# 2068 (TQ4WS azide, click chemistry)
Cat# 2069 (TQ4WS alkyne, click chemistry)
Tide Quencher™ 5 (TQ5)~670
  • Alternative to QSY® 21 and BHQ®-3
  • Best paired with Tide Fluor™ 5 (TF5)
  • Excellent FRET efficiency with Cy5®, DyLight® 649 and Alexa Fluor®647
Cat# 2077 & 2078 (TQ5 CPG, OH-reactive)
Tide Quencher™ 5WS (TQ5WS)~670
  • Alternative to QSY® 21 and BHQ®-3
  • Best paired with Tide Fluor™ 5 (TF5)
  • Excellent FRET efficiency with Cy5®, DyLight® 649 and Alexa Fluor®647
Cat# 2075 (TQ5WS acid)
Cat# 2076 (TQ5WS amine)
Cat# 2079 (TQ5WS maleimide, SH-reactive)
Cat# 2081 (TQ5WS SE, NH2-reactive)
Cat# 2082 (TQ5WS azide, click chemistry)
Cat# 2083 (TQ5WS alkyne, click chemistry)
Tide Quencher™ 6WS (TQ6WS)~700
  • Stronger absorption
  • Best paired with Tide Fluor™ 6 (TF6)
  • Better FRET efficiency with Cy5.5®, IRDye® 700 and Alexa Fluor® 680 than other commercial quenchers
Cat# 2090 (TQ6WS acid)
Cat# 2091 (TQ6WS amine)
Cat# 2094 (TQ6WS maleimide, SH-reactive)
Cat# 2096 (TQ6WS SE, NH2-reactive)
Cat# 2097 (TQ6WS azide, click chemistry)
Cat# 2098 (TQ5WS alkyne, click chemistry)