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Assay volumes for various size cell culture plates.
Posted August 6, 2019
Answer
Table 1. Assay volumes for different size cell culture plates.
|
Culture plate
|
Trypsin approx. volume (mL)
| Versene approx. volume (mL) |
Growth medium approx. volume (mL)
|
|
6-well
|
1
| 1 | 1 to 3 |
|
12-well
|
0.4 to 1
| 0.4 to 1 | 1 to 2 |
|
24-well
|
0.2 to 0.3
| 0.2 to 0.3 | 0.5 to 1.0 |
|
48-well
|
0.1 to 0.2
| 0.1 to 0.2 | 0.2 to 0.4 |
|
96-well
|
0.05 to 0.1
| 0.05 to 0.1 | 0.1 to 0.2 |