Assay volumes for various size cell culture plates.

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Accepted Answer

Table 1. Assay volumes for different size cell culture plates.

Culture plate

Trypsin approx. volume (mL)

Versene approx. volume (mL)

Growth medium approx. volume (mL)

6-well

1

1
1 to 3

12-well

0.4 to 1

0.4 to 1
 1 to 2

24-well

0.2  to 0.3

0.2  to 0.3
0.5 to 1.0

48-well

0.1 to 0.2

0.1 to 0.2 
0.2 to 0.4

96-well

0.05 to 0.1

0.05 to 0.1 
0.1 to 0.2

Culture plate	Trypsin approx. volume (mL)	Versene approx. volume (mL)	Growth medium approx. volume (mL)
6-well	1	1	1 to 3
12-well	0.4 to 1	0.4 to 1	1 to 2
24-well	0.2 to 0.3	0.2 to 0.3	0.5 to 1.0
48-well	0.1 to 0.2	0.1 to 0.2	0.2 to 0.4
96-well	0.05 to 0.1	0.05 to 0.1	0.1 to 0.2