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My cells are dying after transfection-what solutions do you recommend?
Posted February 19, 2020
Cell AssayCell CytotoxicityCell Membrane IntegrityCell ViabilityControlsDNAProteinTransfectioncell culturecell damagecell death
Answer
There are many possible reasons for excessive post-transfection cell death, but we recommend these 3 possibilities for initial troubleshooting:
 
  • Run an additional control of DNA without reagent: expressed protein may be toxic
 
  • Check for contamination and treat as necessary, or run procedure with new culture
 
  • Selection antibiotic may be too strong or added too soon after procedure. Test again with lower concentrations and longer interval
 
Additional resources
Transfectamine™ 5000 Transfection ReagentLive or Dead™ Cell Viability Assay KitCell Viability

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