My cells are dying after transfection-what solutions do you recommend?

There are many possible reasons for excessive post-transfection cell death, but we recommend these 3 possibilities for initial troubleshooting:

• Run an additional control of DNA without reagent: expressed protein may be toxic

• Check for contamination and treat as necessary, or run procedure with new culture

• Selection antibiotic may be too strong or added too soon after procedure. Test again with lower concentrations and longer interval