My cells are dying after transfection-what solutions do you recommend?
Posted February 19, 2020
There are many possible reasons for excessive post-transfection cell death, but we recommend these 3 possibilities for initial troubleshooting:
- Run an additional control of DNA without reagent: expressed protein may be toxic
- Check for contamination and treat as necessary, or run procedure with new culture
- Selection antibiotic may be too strong or added too soon after procedure. Test again with lower concentrations and longer interval
Transfectamine™ 5000 Transfection Reagent
Live or Dead™ Cell Viability Assay Kit