AAT Bioquest

My cells are dying after transfection-what solutions do you recommend?

Posted February 19, 2020


There are many possible reasons for excessive post-transfection cell death, but we recommend these 3 possibilities for initial troubleshooting:


  • Run an additional control of DNA without reagent: expressed protein may be toxic


  • Check for contamination and treat as necessary, or run procedure with new culture


  • Selection antibiotic may be too strong or added too soon after procedure. Test again with lower concentrations and longer interval


Additional resources

Transfectamine™ 5000 Transfection Reagent

Live or Dead™ Cell Viability Assay Kit

Cell Viability