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My cells are dying after transfection-what solutions do you recommend?
Posted February 19, 2020

Answer

There are many possible reasons for excessive post-transfection cell death, but we recommend these 3 possibilities for initial troubleshooting:

 

  • Run an additional control of DNA without reagent: expressed protein may be toxic

 

  • Check for contamination and treat as necessary, or run procedure with new culture

 

  • Selection antibiotic may be too strong or added too soon after procedure. Test again with lower concentrations and longer interval