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At what temperature does RNA denature?
Posted December 8, 2020
NucleusDNA and RNAPhysiological Probes
Answer

RNA denaturation happens at 65-70°C when heated for 15 minutes. At high temperature, RNA denaturation happens in <15 minutes. It is always advised to store RNA at -80°C (up to 1 year) and for long duration, RNA samples may also be stored at -20°C as ethanol precipitates.

Additional resources
Removal of RNA by heat treatmentPortelite™ Fluorimetric RNA Quantitation Kit*Optimized for Cytocite™ and Qubit™ Fluorometers*

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