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Can Hoechst be used to stain bacteria?

Posted May 6, 2021


Answer

Yes, Hoechst can be used to stain bacteria, although the staining is dimmer than that seen in mammalian cells. Live as well as dead bacteria can be stained with a Hoechst dilution of 12-15 µg/ml in PBS or 150 mM NaCl for 30 minutes at room temperature. Dead cells stain more brightly than live cells.

Additional resources

Use of hoechst dyes 33258 and 33342 for enumeration of attached and planktonic bacteria

Hoechst 33342 *Ultrapure Grade*

Hoechst 33258 *CAS 23491-45-4*