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Can I fix the cells before or after addition of Calcium detection dyes?
Posted June 12, 2019
calcium indicatorcalciumcalcium fluxCa2+GPCRcalbryteCalcium dyes
Answer

Fixing cells compromises the ability of the cells to retain the dyes inside the cells. We strongly discourage fixing the cells for the calcium detection dyes.

Additional resources
List of Calcium Dyes

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