X
Navigation Menu
Products
Services
Tools
Resources
logo
AAT Bioquest

 
search
logo
AAT Bioquest
Contact us
Company
Telephone:1-408-733-1055
Fax:1-408-733-1304
Hours:Monday to Friday
8:30 - 17:30 PST (GMT-8)
Location:5775 W Las Positas Blvd.
Pleasanton, CA, 94588
USA

Email us
Sales:sales@aatbio.com
Technical Support:support@aatbio.com
Website:websupport@aatbio.com
General Inquiries:info@aatbio.com
Order info
United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

International
Click here to see all available distributors
Products
Services
Tools
Resources
 
search
calculatorCalculator

Can I fix the cells before or after addition of Calcium detection dyes?

Posted June 12, 2019


calcium indicator
calcium
calcium flux
Ca2+
GPCR
calbryte
Calcium dyes
Answer

Fixing cells compromises the ability of the cells to retain the dyes inside the cells. We strongly discourage fixing the cells for the calcium detection dyes.

Additional resources

List of Calcium Dyes

Related questions
What is the molar extinction coefficient of RNA?
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Location: Home / Frequently Asked Questions (FAQ) / Can I fix the cells before or after addition of Calcium detection dyes?
AboutPrivacyTerms of UseTerms of SalesDistributors
Copyright © 2024 AAT Bioquest, Inc. All Rights Reserved.