Search by catalog number, product name, application...
Search by catalog number, product name, application...
Contact Us
Place Order
View Cart
My Account
Products
Services
Resources
Selection Guides
About
Can I fix the cells before or after addition of Calcium detection dyes?
Posted June 12, 2019
calcium indicator
calcium
calcium flux
Ca2+
GPCR
calbryte
Calcium dyes
Answer
Fixing cells compromises the ability of the cells to retain the dyes inside the cells. We strongly discourage fixing the cells for the calcium detection dyes.
Additional resources
List of Calcium Dyes
Related questions
What is the molar extinction coefficient of RNA?
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
What is HHBS?
Home
/
Frequently Asked Questions (FAQ)
/
Can I fix the cells before or after addition of Calcium detection dyes?
About
Privacy
Terms of Use
Terms of Sale
Distributors
Copyright © 2026 AAT Bioquest, Inc. All Rights Reserved.