logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
Posted April 1, 2019

I wonder how many wells can be measured in a run, without loss of signal in the last wells distorting a dose curve for example.

calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator
Answer

The endpoint assay is appropriate to determine the calcium response from a full 96-well plate if the whole plate is read immediately after the addition of the calcium flux stimulant.

Additional resources
Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit *Optimized for microplate reader*

When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?What is the concentration of calcium inside cells?Why do CHO cells require the use of probenecid in calcium assays?Which fluorescent calcium indicators are compatible with GFP positive cells?