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AAT Bioquest

Can polyclonal antibodies that are generated against tagged proteins cross-react?

Posted April 24, 2020


Answer

For antibodies that are generated using tagged proteins, cross-reaction is very likely, since the antibodies will recognize not only the protein of interest but also its tags. It may be helpful to remove the tags from the protein prior to injection; this will help you obtain antibodies that are more specific for the protein of interest.