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Do I need to deproteinize my samples before using Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)?
Posted August 15, 2018
GSHGSSGGSH/GSSG ratiofluorimetricfluorescence microplate reader
Answer

If you are finding inconsistent or unexpected results, then one solution may be to deproteinize samples before using the assay.

Additional resources
Amplite™ Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1? How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells? How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells? Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?Can I use Triton X-100 as my lysis buffer for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)? Can I use X buffer to lysate/prepare my samples?