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Do I need to deproteinize my samples before using Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)?

Posted August 15, 2018


GSH
GSSG
GSH/GSSG ratio
fluorimetric
fluorescence microplate reader
Answer

If you are finding inconsistent or unexpected results, then one solution may be to deproteinize samples before using the assay.

Additional resources

Amplite™ Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Related questions
Can I use Triton X-100 as my lysis buffer for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)? Can I use X buffer to lysate/prepare my samples?
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?
Location: Home / Frequently Asked Questions (FAQ) / Do I need to deproteinize my samples before using Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)?
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