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How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
Posted January 22, 2020
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader
Answer

While we do not provide protocols for specific cell types, we are happy to answer any application specific questions with regards to our Amplite® Fluorimetric Glutathione Assay and Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay kits. Please contact our technical support team at support@aatbio.com or 1-800-990-8053.

Additional resources
Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1? Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells? Can I use Triton X-100 as my lysis buffer for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)? Can I use X buffer to lysate/prepare my samples?How does the Amplite® Fluorimetric Glutathione GSH/GSSG Ratio assay work? What is the assay actually quantifying?