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How does the Amplite® Fluorimetric Glutathione GSH/GSSG Ratio assay work? What is the assay actually quantifying?
Posted April 2, 2019
GSSGGSHGSH/GSSG ratiocell-permeable probesfluorimetricglutathione
Answer

Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit Green Fluorescence (Cat# 10056) contains a probe which reacts ONLY with the reduced form of glutathione (ie. GSH) and not with GSSG. By measuring the GSH concentration before reduction and measuring the Total GSH concentration after reduction, users can calculate the GSSG concentration as well as the GSH/GSSG ratio. For specific equations and procedures, please consult our sample protocol.

Additional resources
Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1? How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells? Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells? Why is the Total GSH standard solution concentration equal to twice that of the GSSG standard solution concentration? Why is [GSSG] equal to ([Total GSH] - [GSH]) DIVIDED by 2?