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Why is the Total GSH standard solution concentration equal to twice that of the GSSG standard solution concentration? Why is [GSSG] equal to ([Total GSH] - [GSH]) DIVIDED by 2?
Posted August 10, 2018
GSHGSSGglutathioneGSH/GSSG ratiofluorimetricfluorescent plate reader
Answer

After reduction, 1 mole of GSSG becomes 2 moles of GSH. Thus, if the initial GSSG concentration is 1 µM, the GSH concentration after reduction is 2 µM. Conversely, to determine [GSSG] from [Total GSH], you have to divide by two to account for the GSSG to GSH molar ratio: 1 GSSG ⟶ 2 GSH.  

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