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AAT Bioquest

Do I need to permeabilize cells for Hoechst staining?

Posted May 6, 2021


Answer

Hoechst dyes are cell-permeable so there is no need to permeabilize them for Hoechst staining.  

Additional resources

Permeabilization of cell membranes

Hoechst 33342 *Ultrapure Grade*

Hoechst 33258 *CAS 23491-45-4*