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Do I need to permeabilize cells for Hoechst staining?
Posted May 6, 2021
NuleusDNA and RNAPhysiological Probes
Answer

Hoechst dyes are cell-permeable so there is no need to permeabilize them for Hoechst staining.  

Additional resources
Permeabilization of cell membranesHoechst 33342 *Ultrapure Grade*Hoechst 33258 *CAS 23491-45-4*

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