AAT Bioquest

What affects PCR efficiency?

Posted November 25, 2020


The following parameters can adversely affect your PCR efficiency:

  • Poorly designed PCR primers
  • PCR inhibitors are present in your samples
  • Inconsistent pipetting techniques leading to inaccurate sample and reagent pipetting
  • Standard curve was analyzed incorrectly
Additional resources

pcrEfficiency: a Web tool for PCR amplification efficiency prediction

ROX Reference Dye for Real-Time PCR

PCR Detection of Viral DNA/RNA

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity*