What affects PCR efficiency?
Posted November 25, 2020
Answer
The following parameters can adversely affect your PCR efficiency:
- Poorly designed PCR primers
- PCR inhibitors are present in your samples
- Inconsistent pipetting techniques leading to inaccurate sample and reagent pipetting
- Standard curve was analyzed incorrectly
Additional resources
pcrEfficiency: a Web tool for PCR amplification efficiency prediction
ROX Reference Dye for Real-Time PCR
PCR Detection of Viral DNA/RNA
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity*