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What affects PCR efficiency?
Posted November 25, 2020
NucleusDNA and RNAPhysiological Probes
Answer

The following parameters can adversely affect your PCR efficiency:

  • Poorly designed PCR primers
  • PCR inhibitors are present in your samples
  • Inconsistent pipetting techniques leading to inaccurate sample and reagent pipetting
  • Standard curve was analyzed incorrectly
Additional resources
pcrEfficiency: a Web tool for PCR amplification efficiency predictionROX Reference Dye for Real-Time PCRReverse Transcription PCR (RT-PCR)Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity*

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