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How do I calculate FRET efficiency?
Posted March 17, 2021
Chemical ReagentsPeptide and Oligonucleotide LabelingFörster Resonance Energy Transfer (FRET)
Answer

FRET efficiency is calculated as the ratio: Fa / (Fd + Fa), where Fa is the acceptor emission and Fd is the donor emission.

In order to calculate the FRET efficiency, you need the integrated intensity of the acceptor emission spectrum and that of the donor emission spectrum, both upon excitation of the donor only.

Additional resources
Measurement of FRET Efficiency and Ratio of Donor to Acceptor Concentration in Living CellsFMOC-Lys(5-TAMRA)-OHPeptide and Oligonucleotide Labeling

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