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How do I calculate concentration from absorbance?
Posted December 2, 2020
Answer
Use the Beer-Lambert Law to calculate the concentration of a solution from the absorbance measurements.
Rearrange the formula by solving for c:
Where:
A = the molar absorptivity
ε = the molar extinction coefficient
l = fixed path length
c = the concentration
ε = the molar extinction coefficient
l = fixed path length
c = the concentration