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AAT Bioquest

How do I calculate concentration from absorbance?

Posted December 2, 2020


Answer

Use the Beer-Lambert Law to calculate the concentration of a solution from the absorbance measurements. 

Rearrange the formula by solving for c: 

Where:

= the molar absorptivity
ε = the molar extinction coefficient
= fixed path length
= the concentration

 

 

Additional resources

Spectrophotometric determination of protein concentration.

Absorption Spectrum Viewer