How do you evaluate the transfection efficiency? | AAT Bioquest

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How do you evaluate the transfection efficiency?

Posted May 18, 2020

Chemical Reagents DNA and RNA Lipofectamine Lipofectamine 2000 Lipofectamine 3000 Nucleic Acids Transfection Transfection Reagents

Answer

Transfection efficiency is the percentage of cells that are transfected compared to the entire population. It usually can be evaluated by measuring the expression of reporter genes on the transfection plasmids, such as GFP.

Additional resources

Transfectamine™ 5000 Transfection Reagent Sandbichler, A. M., Aschberger, T., & Pelster, B. (2013). A Method to Evaluate the Efficiency of Transfection Reagents in an Adherent Zebrafish Cell Line. BioResearch Open Access, 2(1), 20–27. doi:10.1089/biores.2012.0287 Transfection Reagents

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