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AAT Bioquest

How does transfection with lipofectamine 2000 work?

Posted April 17, 2020


Answer

Liposome transfection is a technique of inserting genetic material into cells using liposomes. In liposome transfection, cationic lipids are used to form liposomes, which take up nucleic acids. These nucleic acids can be DNA or siRNA. The positive charge of the liposomes and negative charge of the nucleic acids allow the two to form a complex, which can then enter the cell through endocytosis.

Additional resources

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