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How is Forster distance calculated?
Posted April 30, 2021
Answer
The Förster distance represents the molecular separation at which energy transfer is 50-percent efficient. Forster distance (R0)for each donor-acceptor pair is calculated using the formula:
R0= 0.2108 [κ2 ΦDn-4J]1/6 nm.
'κ' is the orientation factor between two molecules, 'ΦD' is the quantum yield of donor fluorescence (without acceptor), 'n' is the refraction index of the medium surrounding the FRET pair, and 'J' is the overlap integral. Forster distance is presented in nanometers for each FRET pair.
R0= 0.2108 [κ2 ΦDn-4J]1/6 nm.
'κ' is the orientation factor between two molecules, 'ΦD' is the quantum yield of donor fluorescence (without acceptor), 'n' is the refraction index of the medium surrounding the FRET pair, and 'J' is the overlap integral. Forster distance is presented in nanometers for each FRET pair.