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What color does Cy5 fluoresce?
Posted July 1, 2021
Answer
Cy5 fluoresces in the far-red region.
Cy5 is a fluorescent dye belonging to the Cyanine family of synthetic polymethine dyes. Its absorption wavelength peaks around 646-649 nm, and emission maximum is around 664-670 nm. Cy5 is widely used to label DNA oligos for use as hybridization probes in other applications, such as Fluorescent In-Situ Hybridization (FISH).
Additional resources
Direct eye visualization of Cy5 fluorescence for immunocytochemistry and in situ hybridization