AAT Bioquest

How are recombinant antibodies made?

Posted June 29, 2022


Recombinant antibodies are monoclonal antibodies that are generated in vitro using synthetic genes. Recombinant antibody production uses recombinant DNA technology. This involves recovering antibody genes from source cells and cloning the antibody-coding genes into high-yield expression vectors, which are then transfected into host cells for expression. This is different from the production of conventional monoclonal antibodies, which involves immunizing animals with immunogens to provoke immune responses. Production of recombinant antibodies is done without cultivating hybridomas or immunizing animals. A recombinant monoclonal antibody is made up of identical antibody chains that target the same epitope. Advantages of recombinant monoclonal antibodies include improved sensitivity, increased reproducibility, confirmed specificity and guaranteed continuous supply.  

Additional resources

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