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How is cytosolic calcium tested?
Posted July 28, 2021
Calcium IndicatorsIntracellular Probes
Answer
Detecting changes in cytosolic calcium is normally performed using a live cell imaging set up with calcium binding dyes, such as Fura-2 and Fluo-3, that exhibit either an increase in fluorescence intensity or a shift in the emission wavelength upon calcium binding.
Additional resources
Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia CellsCalcium Indicators

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