AAT Bioquest

How is cytosolic calcium tested?

Posted July 28, 2021


Detecting changes in cytosolic calcium is normally performed using a live cell imaging set up with calcium binding dyes, such as Fura-2 and Fluo-3, that exhibit either an increase in fluorescence intensity or a shift in the emission wavelength upon calcium binding.

Additional resources

Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells

Calcium Indicators