AAT Bioquest

I am performing an ELISA using biotinylated goat secondary antibodies, can you recommend a working concentration?

Posted January 2, 2020


AAT Bioquest offers two biotinylated goat secondary antibodies:

  • Biotinylated Goat Anti-Mouse IgG (Cat No. 16729)
  • Biotinylated Goat Anti-Rabbit IgG (Cat No. 16794)

The following table provides various working concentrations to use in each application. It is important to note that the following are recommended concentrations. For best results, the optimal biotinylated secondary working concentration must be determined experimentally for each assay and is usually determined by using a series of dilutions in a titration experiment.

ApplicationRecommended Working Concentration
ELISA20 ∼ 100 ng/mL
IF1 ∼ 10 µg/mL
IHC1 ∼ 10 µg/mL
Western blot (WB)0.2 ∼ 1 µg/mL
Additional resources

Biotinylated goat anti-mouse IgG

Biotinylated goat anti-rabbit IgG