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Detection of low abundance of target protein?
Posted December 23, 2019
How do I detect very low- abundance antigen? What are the best amplification system for signal improvement?
Biotin
Streptavidin
PSA
Signal Amplification
Tyramide
TSA
Answer
A common method of amplifying antibody detection is biotin-streptavidin detection. This will amplify signal by 2-8 fold. Another option is to use Power Styramide™ Signal Amplification (PSA™) system. This will amplify signal by 10-50 times.
Links:
Power Styramide™ Signal Amplification (PSA™)
Biotin and Streptavidin
Additional resources
Power Styramide™ Signal Amplification (PSA™)
Biotin and Streptavidin
Related questions
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How many biotin molecules can streptavidin bind?
I am performing an ELISA using biotinylated goat secondary antibodies, can you recommend a working concentration?
What is a Sandwich ELISA?
What is an Indirect ELISA?
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Detection of low abundance of target protein?
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