AAT Bioquest

Detection of low abundance of target protein?

Posted December 23, 2019

How do I detect very low- abundance antigen? What are the best amplification system for signal improvement?


A common method of amplifying antibody detection is biotin-streptavidin detection. This will amplify signal by 2-8 fold. Another option is to use Power Styramide™ Signal Amplification (PSA™) system. This will amplify signal by 10-50 times.


Additional resources

Power Styramide™ Signal Amplification (PSA™)

Biotin and Streptavidin