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I want to include a reduced control in the Total Thiol Quantitation Assay (Cat#5524). I am using TCEP to reduce the protein, but the background signal is high, how can I remove TCEP in solution?
Posted June 13, 2019
Answer
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins. However, there are numerous reports that TCEP is not compatible with downstream assays. Use ReadiUse™ TCEP removing buffer, which can effectively remove residual TCEP in the reaction mixture and is compatible with the followed maleimide reactions.