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In IHC what should be incubation time for primary antibody and secondary antibody ?
Posted April 17, 2020
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies
Answer
In IHC, the primary antibody should be incubated overnight at 4°C, and the secondary antibodies should be incubated for 1-2 hours at room temperature.
Additional resources
Immunohistochemistry (IHC)

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