AAT Bioquest

Is Hoechst cytotoxic?

Posted May 5, 2021


Hoechst stains are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Hoechst dyes induce lower cytotoxicity as compared to other dyes. The staining is very stable and non-toxic to live cells for several days or longer.

Additional resources

Cytotoxicity, Mutagenicity and DNA damage by Hoechst 33342

Hoechst 33342 *Ultrapure Grade*