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AAT Bioquest

Is IgG a secondary antibody?

Posted September 21, 2021


Answer

IgG is not a secondary antibody. Anti-IgG is secondary antibody.

A primary antibody is generally IgG isotype. You will need an anti-IgG H&L (Heavy & Light chains) antibody as a secondary antibody, which reacts with both the heavy and light chains of the primary IgG antibody. In addition, this anti-IgG H&L antibody also reacts with other immunoglobulin classes such as IgM and IgA of the species of the primary antibody, because all immunoglobulins in a given species have the same light chains.

Additional resources

The history of IgG glycosylation and where we are now

iFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG