Which enzyme is most often used in blotting procedures? | AAT Bioquest

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Which enzyme is most often used in blotting procedures?

Posted May 2, 2024

Antibodies and Proteomics Enzymes Horseradish Peroxidase (HRP) and Poly-HRP Immunohistochemistry (IHC)Secondary Antibodies

Answer

Horse-radish peroxidase (HRP) or phosphatase-labeled secondary antibodies are the most popular enzymes used in blotting procedures because they generate colorful products when combined with the substrate during detection.

Additional resources

Overview of Western Blotting Western Blotting Assays Horseradish Peroxidase (HRP) and Poly-HRP MegaWox™ polyHRP-Goat Anti-Mouse IgG Conjugate

Why is horseradish peroxidase the enzyme that is used most often in blotting procedures?How do I know if an antibody is primary or secondary?Why is an HRP conjugated secondary antibody used in western blotting?What are the labels used for direct immunohistochemistry (IHC) methods?How do I choose secondary antibodies?