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Why is horseradish peroxidase the enzyme that is used most often in blotting procedures?
Posted May 2, 2024
Antibodies and ProteomicsEnzymesHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)Secondary Antibodies
Answer

HRP is preferred due to its stability, its cost-effectiveness, and ability to be easily conjugated to other molecules. HRP is also smaller in size compared to phosphatase-labeled secondary antibodies. 

Additional resources
Overview of Western BlottingHorseradish Peroxidase (HRP) and Poly-HRP MegaWox™ polyHRP-Goat Anti-Mouse IgG Conjugate

Which enzyme is most often used in blotting procedures?How do I know if an antibody is primary or secondary?Why is an HRP conjugated secondary antibody used in western blotting?What are the common enzymes used as labels for ELISA assays?How do I choose secondary antibodies?