What antibody concentration is recommended for intracellular staining using flow cytometry? | AAT Bioquest

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What antibody concentration is recommended for intracellular staining using flow cytometry?

Posted May 9, 2020

Antibodies and Proteomics Antibody and Protein Labeling Flow Cytometry Reagents Intracellular Probes Spectral Flow Cytometry flow cytometry

Answer

The optimal antibody concentration to use may vary depending on the experiment, but a good starting point to use is a concentration of 1 µg/ml. The best method would be to test various concentrations of antibodies to figure out the best concentration for your experiment.

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