Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5? | AAT Bioquest

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Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5?

Posted May 9, 2020

Antibodies and Proteomics Bioconjugation Antibody and Protein Labeling Cluster of Differentiation (CD Antibodies)Flow Cytometry Reagents Immunohistochemistry (IHC)PE and APC Spectral Flow Cytometry

Answer

Yes, there is an overlap in the emission spectra of PerCP-Cy5.5 and PE-Cy5. Thus, these two dyes should not be used simultaneously.

Additional resources

PerCP/Cy5.5 Anti-human CD8 Antibody *HIT8a*PE-Cy5 Tandem PE/Cy5 Anti-human CD4 Antibody *HIT4a*

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