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What is the right DAPI concentration to use in a flow cytometry experiment?
Posted April 24, 2020
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Structure and OrganellesCellular ProcessesDAPIFlow Cytometry ReagentsSpectral Flow Cytometry
Answer
It depends on the cells used, but a concentration of anywhere from 0.1-1 ug/ml should generally be sufficient. You can titrate DAPI to determine the concentration best suited for your cell type.
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