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What is the right DAPI concentration to use in a flow cytometry experiment?
Posted April 24, 2020
Answer
It depends on the cells used, but a concentration of anywhere from 0.1-1 ug/ml should generally be sufficient. You can titrate DAPI to determine the concentration best suited for your cell type.
Additional resources
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*