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Is it possible to use DAPI and PI simultaneously?
Posted May 9, 2020
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDAPIFluorescenceImmunohistochemistry (IHC)Propidium iodide (PI)
Answer
Yes, it is completely possible to use DAPI and PI (propidium iodide) simultaneously, since the two dyes bind differently to DNA. DAPI binds to the minor grooves of DNA, while PI is an intercalator.
Additional resources
Cummings, B. S., Wills, L. P., & Schnellmann, R. G. (2012). Measurement of cell death in mammalian cells. Current protocols in pharmacology, 56(1), 12-8. Doležel, J., Sgorbati, S., & Lucretti, S. (1992). Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiologia plantarum, 85(4), 625-631. DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*Propidium iodide *CAS 25535-16-4*